Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects

H Li, Y Yang, W Hong, M Huang, M Wu… - Signal transduction and …, 2020 - nature.com
Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

[HTML][HTML] Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy

MF Rasul, BM Hussen, A Salihi, BS Ismael, PJ Jalal… - Molecular Cancer, 2022 - Springer
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-associated protein
9) shows the opportunity to treat a diverse array of untreated various genetic and …

[HTML][HTML] Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing

T Wei, Q Cheng, YL Min, EN Olson… - Nature communications, 2020 - nature.com
CRISPR-Cas9 has emerged as a powerful technology that relies on Cas9/sgRNA
ribonucleoprotein complexes (RNPs) to target and edit DNA. However, many therapeutic …

CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy

D Rosenblum, A Gutkin, R Kedmi, S Ramishetti… - Science …, 2020 - science.org
Harnessing CRISPR-Cas9 technology for cancer therapeutics has been hampered by low
editing efficiency in tumors and potential toxicity of existing delivery systems. Here, we …

A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing

G Chen, AA Abdeen, Y Wang, PK Shahi… - Nature …, 2019 - nature.com
Delivery technologies for the CRISPR-Cas9 (CRISPR, clustered regularly interspaced short
palindromic repeats) gene editing system often require viral vectors, which pose safety …

Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications

C Liu, L Zhang, H Liu, K Cheng - Journal of controlled release, 2017 - Elsevier
The CRISPR-Cas9 genome-editing system is a part of the adaptive immune system in
archaea and bacteria to defend against invasive nucleic acids from phages and plasmids …

Surface chemistry of gold nanoparticles for health-related applications

J Zhang, L Mou, X Jiang - Chemical Science, 2020 - pubs.rsc.org
Functionalization of gold nanoparticles is crucial for the effective utilization of these materials
in health-related applications. Health-related applications of gold nanoparticles rely on the …

Endosomal escape and delivery of CRISPR/Cas9 genome editing machinery enabled by nanoscale zeolitic imidazolate framework

SK Alsaiari, S Patil, M Alyami… - Journal of the …, 2018 - ACS Publications
CRISPR/Cas9 is a combined protein (Cas9) and an engineered single guide RNA (sgRNA)
genome editing platform that offers revolutionary solutions to genetic diseases. It has …

Gene therapy for drug-resistant glioblastoma via lipid-polymer hybrid nanoparticles combined with focused ultrasound

Q Yang, Y Zhou, J Chen, N Huang… - International journal of …, 2021 - Taylor & Francis
Background Therapy for glioblastoma (GBM) has always been very challenging, not only
because of the presence of the blood-brain barrier (BBB) but also due to susceptibility to …

[HTML][HTML] CRISPR-Cas systems: Overview, innovations and applications in human disease research and gene therapy

Y Xu, Z Li - Computational and structural biotechnology journal, 2020 - Elsevier
Genome editing is the modification of genomic DNA at a specific target site in a wide variety
of cell types and organisms, including insertion, deletion and replacement of DNA, resulting …