Current advances in experimental and computational approaches to enhance CAR T cell manufacturing protocols and improve clinical efficacy

AS Colina, V Shah, RK Shah, T Kozlik… - Frontiers in Molecular …, 2024 - frontiersin.org
Since the FDA's approval of chimeric antigen receptor (CAR) T cells in 2017, significant
improvements have been made in the design of chimeric antigen receptor constructs and in …

Microengineered in vitro CAR T cell screens and assays

J Kim, SN Thomas - Cell Systems, 2024 - cell.com
Established and emergent microengineered in vitro systems enable the evaluation of
chimeric antigen receptor (CAR) T cell product purity, avidity, and functionality. Here, we …

In vitro encapsulation and expansion of T and CAR-T cells using 3D synthetic thermo-responsive matrices

GD Lizana-Vasquez, J Mendez-Vega… - RSC …, 2024 - pubs.rsc.org
Suspension cell culture and rigid commercial substrates are the most common methods to
clinically manufacture therapeutic CAR-T cells ex vivo. However, suspension culture and …

In Vitro Assessment of Thermo‐Responsive Scaffold as a 3D Synthetic Matrix for CAR‐T Potency Testing Against Glioblastoma Spheroids

GD Lizana‐Vasquez… - … Research Part A, 2025 - Wiley Online Library
Chimeric antigen receptor (CAR) T cell immunotherapy has demonstrated exceptional
efficacy against hematological malignancies, but notably less against solid tumors. To …