Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

As manufacturing processes and development of new synthetic compounds increase to
keep pace with the expanding global demand, environmental health, and the effects of …

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

Introduction Embryogenesis is thought to be directed by a small number of signaling
pathways with most if not all embryonic signals having been identified. However, the …

The contrast and resolution of images obtained with optical microscopes can be improved
by deconvolution and computational fusion of multiple views of the same sample, but these …

Optimal image quality in light-sheet microscopy requires a perfect overlap between the
illuminating light sheet and the focal plane of the detection objective. However, mismatches …

The in-vivo investigation of highly dynamic biological samples, for example the beating
zebrafish heart, requires high-speed volume imaging techniques. Light-sheet microscopy is …

Advances in imaging and cell-labeling techniques have greatly enhanced our
understanding of developmental and neurobiological processes. Among vertebrates …