Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …

Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes …

Counting molecules in complexes is challenging, even with super-resolution microscopy.
Here, we use the programmable and specific binding of dye-labeled DNA probes to count …

The genomes of multicellular organisms are extensively folded into 3D chromosome
territories within the nucleus. Advanced 3D genome-map** methods that combine …

Stochastic optical reconstruction microscopy (STORM) allows wide-field imaging with single-
molecule resolution by calculating the coordinates of individual fluorophores from the …

Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence
microscope without requiring high laser powers or specialized fluorophores. However, the …

Protein clustering is a hallmark of genome regulation in mammalian cells. However, the
dynamic molecular processes involved make it difficult to correlate clustering with functional …

Methods for imaging and tracking single molecules conjugated with fluorescent probes,
called single-molecule tracking (SMT), are now providing researchers with the …

Nuclear export of mRNAs is a crucial step in the regulation of gene expression, linking
transcription in the nucleus to translation in the cytoplasm. Although important components …