Expansion microscopy: principles and uses in biological research

AT Wassie, Y Zhao, ES Boyden - Nature methods, 2019 - nature.com
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …

Expansion microscopy: super-resolution imaging with hydrogels

S Truckenbrodt - Analytical Chemistry, 2023 - ACS Publications
Guidelines, Not Rules 14 Chemical Principles of Accessing Biomolecules in Expansion
Microscopy 14 Accessing Proteins 14 Protein Anchoring with Indirect Readout 14 Protein …

Expansion microscopy: protocols for imaging proteins and RNA in cells and tissues

SM Asano, R Gao, AT Wassie… - Current protocols in …, 2018 - Wiley Online Library
Expansion microscopy (ExM) is a recently developed technique that enables nanoscale‐
resolution imaging of preserved cells and tissues on conventional diffraction‐limited …

X10 expansion microscopy enables 25‐nm resolution on conventional microscopes

S Truckenbrodt, M Maidorn, D Crzan, H Wildhagen… - EMBO …, 2018 - embopress.org
Expansion microscopy is a recently introduced imaging technique that achieves super‐
resolution through physically expanding the specimen by~ 4×, after embedding into a …

A practical guide to optimization in X10 expansion microscopy

S Truckenbrodt, C Sommer, SO Rizzoli, JG Danzl - Nature protocols, 2019 - nature.com
Expansion microscopy is a relatively new approach to super-resolution imaging that uses
expandable hydrogels to isotropically increase the physical distance between fluorophores …

A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy

R Gao, CC Yu, L Gao, KD Piatkevich, RL Neve… - Nature …, 2021 - nature.com
Expansion microscopy (ExM) physically magnifies biological specimens to enable
nanoscale-resolution imaging using conventional microscopes. Current ExM methods …

[HTML][HTML] Expansion microscopy: a powerful nanoscale imaging tool for neuroscientists

BR Gallagher, Y Zhao - Neurobiology of Disease, 2021 - Elsevier
One of the biggest unsolved questions in neuroscience is how molecules and neuronal
circuitry create behaviors, and how their misregulation or dysfunction results in neurological …

Scalable and isotropic expansion of tissues with simply tunable expansion ratio

HE Park, D Choi, JS Park, C Sim, S Park… - Advanced …, 2019 - Wiley Online Library
Tissue expansion techniques physically expand swellable gel‐embedded biological
specimens to overcome the resolution limit of light microscopy. As the benefits of expansion …

Tissue clearing and imaging methods for cardiovascular development

H Kolesová, V Olejníčková, A Kvasilová… - Iscience, 2021 - cell.com
Tissue imaging in 3D using visible light is limited and various clearing techniques were
developed to increase imaging depth, but none provides universal solution for all tissues at …

[HTML][HTML] SYNAPSE: An international roadmap to large brain imaging

APJ Stampfl, Z Liu, J Hu, K Sawada, H Takano… - Physics Reports, 2023 - Elsevier
Since 2020, synchrotron radiation facilities in several Asia-Pacific countries have been
collaborating in a major project called “SYNAPSE”(Synchrotrons for Neuroscience: an Asia …