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Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility
Induced pluripotent stem cell (iPSC) technologies have provided in vitro models of
inaccessible human cell types, yielding new insights into disease mechanisms especially for …
inaccessible human cell types, yielding new insights into disease mechanisms especially for …
Single-cell transcriptomics meets lineage tracing
Reconstructing lineage relationships between cells within a tissue or organism is a long-
standing aim in biology. Traditionally, lineage tracing has been achieved through the …
standing aim in biology. Traditionally, lineage tracing has been achieved through the …
[HTML][HTML] An epigenetic barrier sets the timing of human neuronal maturation
The pace of human brain development is highly protracted compared with most other
species,,,,,–. The maturation of cortical neurons is particularly slow, taking months to years to …
species,,,,,–. The maturation of cortical neurons is particularly slow, taking months to years to …
To facilitate scalable profiling of single cells, we developed split-pool ligation-based
transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that …
transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that …
Lagre Referanse Sitert av 1316 BA Biddy, W Kong, K Kamimoto, C Guo, SE Waye… - Nature, 2018 - nature.com
Direct lineage reprogramming involves the conversion of cellular identity. Single-cell
technologies are useful for deconstructing the considerable heterogeneity that emerges …
technologies are useful for deconstructing the considerable heterogeneity that emerges …
Functional modules of the proteostasis network
Cells invest in an extensive network of factors to maintain protein homeostasis (proteostasis)
and prevent the accumulation of potentially toxic protein aggregates. This proteostasis …
and prevent the accumulation of potentially toxic protein aggregates. This proteostasis …
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information
A goal of single-cell genome-wide profiling is to reconstruct dynamic transitions during cell
differentiation, disease onset and drug response. Single-cell assays have recently been …
differentiation, disease onset and drug response. Single-cell assays have recently been …
Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells
Cerebral organoids exhibit broad regional heterogeneity accompanied by limited cortical
cellular diversity despite the tremendous upsurge in derivation methods, suggesting …
cellular diversity despite the tremendous upsurge in derivation methods, suggesting …
Concepts and limitations for learning developmental trajectories from single cell genomics
Single cell genomics has become a popular approach to uncover the cellular heterogeneity
of progenitor and terminally differentiated cell types with great precision. This approach can …
of progenitor and terminally differentiated cell types with great precision. This approach can …