Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

Many proteins contain large structurally disordered regions or are entirely disordered under
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …

Liquid-liquid phase-separated (LLPS) states are key to compartmentalizing components in
the absence of membranes; however, it is unclear whether LLPS condensates are actively …

In response to double strand breaks (DSB), repair proteins accumulate at damaged sites,
forming membrane-less sub-compartments or foci. Here we explored the physical nature of …

CRISPR-Cas9 is widely used in genomic editing, but the kinetics of target search and its
relation to the cellular concentration of Cas9 have remained elusive. Effective target search …

Protein diffusion is crucial for understanding the formation of protein complexes in vivo and
has been the subject of many fluorescence microscopy studies in cells; however, such …

Recent studies have shown that RNA polymerase (RNAP) is organized into distinct clusters
in Escherichia coli and Bacillus subtilis cells. Spatially organized molecular components in …

Liquid droplets of a host protein, formed by liquid–liquid phase separation, recruit guest
proteins and provide functional fields. Recruitment into p53 droplets is similar between …

Bacterial transcription by RNA polymerase (RNAP) is spatially organized. RNAPs
transcribing highly expressed genes locate in the nucleoid periphery, and form clusters in …

The rise in fluorescence-based imaging techniques over the past 3 decades has improved
the ability of researchers to scrutinize live cell biology at increased spatial and temporal …