Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Nanomaterial-microorganism hybrid systems (NMHSs), integrating semiconductor
nanomaterials with microorganisms, present a promising platform for broadband solar …

Directed evolution is one of the most powerful tools for protein engineering and functions by
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …

Synthetic biology provides a new paradigm for life science research (“build to learn”) and
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …

Highlights•Biofuels represent valuable alternatives to fossil fuels to reduce net CO 2
emission.•S. cerevisiae is a widely used chassis with many available synthetic biology …

Fatty acids are crucial molecules for most living beings, very well spread and conserved
across species. These molecules play a role in energy storage, cell membrane architecture …

Highlights•The lack of predictive modeling limits biosystems design for biofuel production.•
Automation in build and test accelerates strain engineering for biofuel research.•Microplates …

Abstract Background Fatty acids (FAs) are essential cellular components and play important
roles in various biological processes. Importantly, FAs produced by microorganisms from …

Cytochrome P450 enzymes (P450s) are a superfamily of heme‐thiolate proteins widely
existing in various organisms. Due to their key roles in secondary metabolism, degradation …

Directed evolution is a powerful approach to engineer enzymes via iterative creation and
screening of variant libraries. However, assay development for high-throughput mutant …