Measuring DNA modifications with the comet assay: a compendium of protocols

A Collins, P Møller, G Gajski, S Vodenková… - Nature protocols, 2023 - nature.com
The comet assay is a versatile method to detect nuclear DNA damage in individual
eukaryotic cells, from yeast to human. The types of damage detected encompass DNA …

Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

P Møller, A Azqueta, E Boutet-Robinet, G Koppen… - Nature protocols, 2020 - nature.com
The comet assay is a widely used test for the detection of DNA damage and repair activity.
However, there are interlaboratory differences in reported levels of baseline and induced …

Comet assay: a versatile but complex tool in genotoxicity testing

E Cordelli, M Bignami, F Pacchierotti - Toxicology Research, 2021 - academic.oup.com
The comet assay is a versatile method for measuring DNA strand breaks in individual cells. It
can also be applied to cells isolated from treated animals. In this review, we highlight …

The essential comet assay: a comprehensive guide to measuring DNA damage and repair

A Azqueta, AR Collins - Archives of toxicology, 2013 - Springer
The comet assay (single cell gel electrophoresis) is the most common method for measuring
DNA damage in eukaryotic cells or disaggregated tissues. The assay depends on the …

Free radicals and antioxidants–quo vadis?

B Halliwell - Trends in pharmacological sciences, 2011 - cell.com
The field of free radicals and antioxidants, or 'redox biology', is fundamental to aerobic life.
Aerobes constantly make reactive species, but modulate their actions by synthesizing …

Genotoxicity of particles from grinded plastic items in Caco-2 and HepG2 cells

M Roursgaard, M Hezareh Rothmann… - Frontiers in public …, 2022 - frontiersin.org
Large plastic litters degrade in the environment to micro-and nanoplastics, which may then
enter the food chain and lead to human exposure by ingestion. The present study explored …

Oxidative stress and inflammation generated DNA damage by exposure to air pollution particles

P Møller, PH Danielsen, DG Karottki, K Jantzen… - … research/Reviews in …, 2014 - Elsevier
Generation of oxidatively damaged DNA by particulate matter (PM) is hypothesized to occur
via production of reactive oxygen species (ROS) and inflammation. We investigated this …

Measuring oxidative damage to DNA and its repair with the comet assay

AR Collins - Biochimica et Biophysica Acta (BBA)-General Subjects, 2014 - Elsevier
Background Single cell gel electrophoresis, or the comet assay, was devised as a sensitive
method for detecting DNA strand breaks, at the level of individual cells. A simple …

Application of the comet assay in human biomonitoring: An hCOMET perspective

A Azqueta, C Ladeira, L Giovannelli… - … Research/Reviews in …, 2020 - Elsevier
The comet assay is a well-accepted biomonitoring tool to examine the effect of dietary,
lifestyle, environmental and occupational exposure on levels of DNA damage in human …

Effect of a wild blueberry (Vaccinium angustifolium) drink intervention on markers of oxidative stress, inflammation and endothelial function in humans with …

P Riso, D Klimis-Zacas, C Del Bo', D Martini… - European journal of …, 2013 - Springer
Abstract Purpose Wild blueberries (WB)(Vaccinium angustifolium) are rich sources of
polyphenols, such as flavonols, phenolic acids and anthocyanins (ACNs), reported to …