Biocatalysis has revolutionized chemical synthesis, providing sustainable methods for
preparing various organic molecules. In enzyme-mediated organic synthesis, most reactions …

The ability to design efficient enzymes from scratch would have a profound effect on
chemistry, biotechnology and medicine. Rapid progress in protein engineering over the past …

De novo enzyme design has sought to introduce active sites and substrate-binding pockets
that are predicted to catalyse a reaction of interest into geometrically compatible native …

The binding and catalytic functions of proteins are generally mediated by a small number of
functional residues held in place by the overall protein structure. Here, we describe deep …

Construction of a scaffold structure that supports a desired motif, conferring protein function,
shows promise for the design of vaccines and enzymes. But a general solution to this motif …

Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

AlphaFold2 and related computational systems predict protein structure using deep learning
and co-evolutionary relationships encoded in multiple sequence alignments (MSAs) …

The computational de novo protein design is increasingly applied to address a number of
key challenges in biomedicine and biological engineering. Successes in expanding …

A protein backbone structure is designable if a substantial number of amino acid sequences
exist that autonomously fold into it,. It has been suggested that the designability of …

In the early 2000s, Tawfik presented his 'New View'on enzyme evolution, highlighting the
role of conformational plasticity in expanding the functional diversity of limited repertoires of …