The quantification of differences between two or more physiological states of a biological
system is among the most important but also most challenging technical tasks in proteomics …

We discuss protein post‐translational modification (PTM) from an information processing
perspective. PTM at multiple sites on a protein creates a combinatorial explosion in the …

Researchers in many biological areas now routinely characterize proteins by mass
spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling …

Stable isotope labeling by amino acids in cell culture (SILAC) is a simple, robust, yet
powerful approach in mass spectrometry (MS)-based quantitative proteomics. SILAC labels …

Posttranslational modifications offer a dynamic way to regulate protein activity, subcellular
localization, and stability. Here we estimate the effect of phosphorylation on protein binding …

Most synaptic inputs are made onto the dendritic tree. Recent work has shown that dendrites
play an active role in transforming synaptic input into neuronal output and in defining the …

The evolution of mass spectrometry–based proteomic technologies has advanced our
understanding of the complex and dynamic nature of proteomes while concurrently …

Plasma membrane depolarization can trigger cell proliferation, but how membrane potential
influences mitogenic signaling is uncertain. Here, we show that plasma membrane …

Kv2. 1 exhibits two distinct forms of localization patterns on the neuronal plasma membrane:
One population is freely diffusive and regulates electrical activity via voltage-dependent K+ …

Gli proteins are transcriptional effectors of the Hedgehog (Hh) pathway in both normal
development and cancer. We describe a program of multisite phosphorylation that regulates …