Controlling synthetic cell-cell communication

JM Smith, R Chowdhry, MJ Booth - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
Synthetic cells, which mimic cellular function within a minimal compartment, are finding wide
application, for instance in studying cellular communication and as delivery devices to living …

Orthogonal light-activated DNA for patterned biocomputing within synthetic cells

D Hartmann, R Chowdhry, JM Smith… - Journal of the American …, 2023 - ACS Publications
Cell-free gene expression is a vital research tool to study biological systems in defined
minimal environments and has promising applications in biotechnology. Develo** …

Bioengineering lipid-based synthetic cells for therapeutic protein delivery

S Siquenique, S Ackerman, A Schroeder… - Trends in …, 2024 - cell.com
Synthetic cells (SCs) offer a promising approach for therapeutic protein delivery, combining
principles from synthetic biology and drug delivery. Engineered to mimic natural cells, SCs …

Precise, Orthogonal Remote-Control of Cell-Free Systems Using Photocaged Nucleic Acids

G Mazzotti, D Hartmann, MJ Booth - Journal of the American …, 2023 - ACS Publications
Cell-free expression of a gene to protein has become a vital tool in nanotechnology and
synthetic biology. Remote-control of cell-free systems with multiple, orthogonal wavelengths …

Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides

D Hartmann, MJ Booth - Communications Chemistry, 2023 - nature.com
Controlling cell-free expression of a gene to protein with non-invasive stimuli is vital to the
future application of DNA nanodevices and synthetic cells. However, little emphasis has …

Bicyclic Caged Morpholino Oligonucleotides for Optical Gene Silencing

S Pattanayak, BR Sarode, A Deiters, JK Chen - ChemBioChem, 2022 - Wiley Online Library
Caged morpholino oligonucleotides (cMOs) are synthetic tools that allow light‐inducible
gene silencing in live organisms. Previously reported cMOs have utilized hairpin, duplex …

Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris

G Naseri, K Prause, HH Hamdo… - Frontiers in Bioengineering …, 2021 - frontiersin.org
The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has become a
powerful eukaryotic expression platform for biopharmaceutical and biotechnological …

Sequence-independent, site-specific incorporation of chemical modifications to generate light-activated plasmids

K Chung, MJ Booth - Chemical Science, 2023 - pubs.rsc.org
Plasmids are ubiquitous in biology, where they are used to study gene-function relationships
and intricate molecular networks, and hold potential as therapeutic devices. Develo** …

A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior

D Ncube, A Tallafuss, J Serafin, J Bruckner… - BMC genomics, 2022 - Springer
Background An essential determinant of a neuron's functionality is its neurotransmitter
phenotype. We previously identified a defined subpopulation of cholinergic neurons …

Light‐Activated Gene Expression System Using a Caging‐Group‐Free Photoactivatable Dye

T Nonomura, M Minoshima… - Angewandte Chemie …, 2024 - Wiley Online Library
Optical regulation of transcription using chemical compounds is an effective strategy to
manipulate gene expression spatiotemporally. Conventional caging approaches with …