Exosome processing and characterization approaches for research and technology development

JJ Lai, ZL Chau, SY Chen, JJ Hill… - Advanced …, 2022 - Wiley Online Library
Exosomes are extracellular vesicles that share components of their parent cells and are
attractive in biotechnology and biomedical research as potential disease biomarkers as well …

Functionalized magnetic nanoparticles for the separation and purification of proteins and peptides

R Eivazzadeh-Keihan, H Bahreinizad, Z Amiri… - TrAC Trends in …, 2021 - Elsevier
Nanoparticles have been widely employed for different biomedical applications due to their
remarkable chemical and physical properties. The surface of nanomaterials can be modified …

State of the art on the separation and purification of proteins by magnetic nanoparticles

TD Le, I Suttikhana, TJ Ashaolu - Journal of Nanobiotechnology, 2023 - Springer
The need for excellent, affordable, rapid, reusable and biocompatible protein purification
techniques is justified based on the roles of proteins as key biomacromolecules. Magnetic …

Recent advances in biosensors for nucleic acid and exosome detection

Z Fu, YC Lu, JJ Lai - Chonnam medical journal, 2019 - synapse.koreamed.org
Biosensors are analytical devices for biomolecule detection that compromise three essential
components: recognition moiety, transducer, and signal processor. The sensor converts …

Salt-induced aggregation of negatively charged gold nanoparticles confined in a polymer brush matrix

S Christau, T Moeller, J Genzer, R Koehler… - …, 2017 - ACS Publications
We report on the salt-induced aggregation of citrate-coated gold nanoparticles (AuNPs)
confined within poly (N-isopropylacrylamide)(PNIPAM) brushes grafted from flat substrates …

Efficient enrichment and analyses of bacteria at ultralow concentration with quick-response magnetic nanospheres

CY Wen, YZ Jiang, XY Li, M Tang, LL Wu… - … applied materials & …, 2017 - ACS Publications
Enrichment and purification of bacteria from complex matrices are crucial for their detection
and investigation, in which magnetic separation techniques have recently show great …

Temperature-responsive magnetic nanoparticles for enabling affinity separation of extracellular vesicles

R Jauregui, S Srinivasan, LN Vojtech… - … applied materials & …, 2018 - ACS Publications
Small magnetic nanoparticles that have surfaces decorated with stimuli-responsive
polymers can be reversibly aggregated via a stimulus, such as temperature, to enable …

Unveiling the Complex World of Extracellular Vesicles: Novel Characterization Techniques and Manufacturing Considerations

JJ Lai, JJ Hill, CY Huang, GC Lee… - Chonnam Medical …, 2024 - pmc.ncbi.nlm.nih.gov
Extracellular vesicles (EVs) function as potent mediators of intercellular communication for
many in vivo processes, contributing to both health and disease related conditions. Given …

Magnetic separation of elastin-like polypeptide receptors for enrichment of cellular and molecular targets

DT Ta, R Vanella, MA Nash - Nano letters, 2017 - ACS Publications
Protein-conjugated magnetic nanoparticles (mNPs) are promising tools for a variety of
biomedical applications, from immunoassays and biosensors to theranostics and drug …

A simple agglutination system for rapid antigen detection from large sample volumes with enhanced sensitivity

Q Wang, N Panpradist, JH Kotnik, RC Willson… - Analytica chimica …, 2023 - Elsevier
Lateral flow assays (LFAs) provide a simple and quick option for diagnosis and are widely
adopted for point-of-care or at-home tests. However, their sensitivity is often limited. Most …