Expanding plastics recycling technologies: chemical aspects, technology status and challenges

H Li, HA Aguirre-Villegas, RD Allen, X Bai… - Green …, 2022 - pubs.rsc.org
Less than 10% of the plastics generated globally are recycled, while the rest are incinerated,
accumulated in landfills, or leak into the environment. New technologies are emerging to …

Mechanism-based design of efficient PET hydrolases

R Wei, G von Haugwitz, L Pfaff, J Mican… - ACS …, 2022 - ACS Publications
Polyethylene terephthalate (PET) is the most widespread synthetic polyester, having been
utilized in textile fibers and packaging materials for beverages and food, contributing …

Particle size reduction of poly (ethylene terephthalate) increases the rate of enzymatic depolymerization but does not increase the overall conversion extent

RK Brizendine, E Erickson, SJ Haugen… - ACS Sustainable …, 2022 - ACS Publications
Enzymatic depolymerization of poly (ethylene terephthalate)(PET) has emerged as a
potential method for PET recycling, but extensive thermomechanical preprocessing to …

Computational scoring and experimental evaluation of enzymes generated by neural networks

SR Johnson, X Fu, S Viknander, C Goldin… - Nature …, 2024 - nature.com
In recent years, generative protein sequence models have been developed to sample novel
sequences. However, predicting whether generated proteins will fold and function remains …

Towards synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase …

OF Brandenberg, OT Schubert, L Kruglyak - Microbial cell factories, 2022 - Springer
Background Biocatalysis offers a promising path for plastic waste management and
valorization, especially for hydrolysable plastics such as polyethylene terephthalate (PET) …

Protein design using structure-based residue preferences

D Ding, AY Shaw, S Sinai, N Rollins, N Prywes… - Nature …, 2024 - nature.com
Recent developments in protein design rely on large neural networks with up to 100s of
millions of parameters, yet it is unclear which residue dependencies are critical for …

A review of cross-disciplinary approaches for the identification of novel industrially relevant plastic-degrading enzymes

J Herbert, AH Beckett, SC Robson - Sustainability, 2022 - mdpi.com
The large-scale global use of plastics has led to one of the greatest environmental issues of
the 21st century. The incredible durability of these polymers, whilst beneficial for a wide …

Towards anchoring evolutionary fitness for protein stability with virtual chemical environment recovery

Z Nie, Y Ma, J Chen, Y Liu, Z Liu, P Yang, F Xu… - bioRxiv, 2024 - biorxiv.org
Protein stability offers valuable insights into protein folding and functionality, making it an
integral component of evolutionary fitness. Previous computational methods possess both …

Exploration of reconstructed PET hydrolase ancestors by biophysical and computational methods

U Bala - 2023 - uwspace.uwaterloo.ca
Ancestral sequence reconstruction (ASR), originally developed for the study of molecular
evolution, has gained attention as a promising method for protein engineering in the recent …