Mass spectrometry using electrospray ionization

GRD Prabhu, ER Williams, M Wilm… - Nature Reviews Methods …, 2023 - nature.com
Applying a high voltage to a liquid passing through a capillary disintegrates the liquid
meniscus to generate a fine spray of charged droplets. As the droplets evaporate, polar …

Studies of biomolecular conformations and conformational dynamics by mass spectrometry

IA Kaltashov, SJ Eyles - Mass spectrometry reviews, 2002 - Wiley Online Library
Abstract I. Introduction 38 A. Protein Conformations and Dynamics 38 B. Protein‐Energy
Landscapes: The Folding Problem and Dynamics in the Native State 38 1. Protein …

[HTML][HTML] Electrospray ionization of large multiply charged species proceeds via Dole's charged residue mechanism

JF De La Mora - Analytica chimica acta, 2000 - Elsevier
The maximum charge zmax on a variety of electrospray ions of globular proteins retaining
their native structure, as well as naturally compact starburst dendrimers is compared to the …

Mechanism of protein aggregation inhibition by arginine: blockage of anionic side chains favors unproductive encounter complexes

YK Ng, L Konermann - Journal of the American Chemical Society, 2024 - ACS Publications
Aggregation refers to the assembly of proteins into nonphysiological higher order structures.
While amyloid has been studied extensively, much less is known about amorphous …

Variable-temperature electrospray ionization for temperature-dependent folding/refolding reactions of proteins and ligand binding

JW McCabe, M Shirzadeh, TE Walker, CW Lin… - Analytical …, 2021 - ACS Publications
Stabilities and structure (s) of proteins are directly coupled to their local environment or
Gibbs free energy landscape as defined by solvent, temperature, pressure, and …

Effects of an electric field on the conformational transition of the protein: a molecular dynamics simulation study

Z Jiang, L You, W Dou, T Sun, P Xu - Polymers, 2019 - mdpi.com
The effect of the electric field on the conformational properties of the protein 1BBL was
investigated by molecular dynamics simulations. Our simulation results clearly capture the …

Acid-Induced Unfolding of Cytochrome c at Different Methanol Concentrations:  Electrospray Ionization Mass Spectrometry Specifically Monitors Changes in the …

L Konermann, DJ Douglas - Biochemistry, 1997 - ACS Publications
The acid-induced denaturation of ferricytochrome c (cyt c) was examined in aqueous
solutions containing different concentrations of methanol by electrospray ionization mass …

Hydrogen/deuterium exchange mass spectrometry with top-down electron capture dissociation for characterizing structural transitions of a 17 kDa protein

J Pan, J Han, CH Borchers… - Journal of the American …, 2009 - ACS Publications
Amide H/D exchange (HDX) mass spectrometry (MS) is widely used for protein structural
studies. Traditionally, this technique involves protein labeling in D2O, followed by acid …

Unfolding of proteins monitored by electrospray ionization mass spectrometry: a comparison of positive and negative ion modes

L Konermann, DJ Douglas - Journal of the American Society for Mass …, 1998 - Springer
Electrospray ionization (ESI) mass spectrometry (MS) in both the positive and negative ion
mode has been used to study protein unfolding transitions of lysozyme, cytochrome c (cyt c) …

Applications of mass spectrometry to metabolomics and metabonomics: Detection of biomarkers of aging and of age‐related diseases

RJ Mishur, SL Rea - Mass spectrometry reviews, 2012 - Wiley Online Library
Every 5 years or so new technologies, or new combinations of old ones, seemingly burst
onto the science scene and are then sought after until they reach the point of becoming …