[HTML][HTML] Recent development of computational cluster analysis methods for single-molecule localization microscopy images

Y Hyun, D Kim - Computational and Structural Biotechnology Journal, 2023 - Elsevier
With the development of super-resolution imaging techniques, it is crucial to understand
protein structure at the nanoscale in terms of clustering and organization in a cell. However …

Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterol

H Kemmoku, K Takahashi, K Mukai, T Mori… - Nature …, 2024 - nature.com
Stimulator of interferon genes (STING) is critical for the type I interferon response to
pathogen-or self-derived DNA in the cytosol. STING may function as a scaffold to activate …

Quantitative Single‐Molecule Electrochemiluminescence Bioassay

W Zhu, J Dong, G Ruan, Y Zhou… - Angewandte Chemie …, 2023 - Wiley Online Library
A single‐molecule electrochemiluminescence bioassay is developed here which allows
imaging and direct quantification of single biomolecules. Imaging single biomolecules is …

A framework for evaluating the performance of SMLM cluster analysis algorithms

DJ Nieves, JA Pike, F Levet, DJ Williamson… - Nature …, 2023 - nature.com
Single-molecule localization microscopy (SMLM) generates data in the form of coordinates
of localized fluorophores. Cluster analysis is an attractive route for extracting biologically …

Recent advances in fluorescent nanoparticles for stimulated emission depletion imaging

L Qi, S Liu, J **, X Yao, L Chen, D Yang, Y Liu… - Biosensors, 2024 - mdpi.com
Stimulated emission depletion (STED) microscopy, as a popular super-resolution imaging
technique, has been widely used in bio-structure analysis and resolving the dynamics of …

Quantitatively lighting up the spatial organization of CD47/SIRPα immune checkpoints on the cellular membrane with single-molecule localization microscopy

Y Wei, M Zhao, T He, N Chen, L Rao, L Chen… - ACS …, 2023 - ACS Publications
Immunotherapy including immune checkpoint inhibition has reinvigorated the current cancer
treatment field. The development of efficient cancer immunotherapies depends on a …

High-precision estimation of emitter positions using Bayesian grou** of localizations

M Fazel, MJ Wester, DJ Schodt, SR Cruz… - Nature …, 2022 - nature.com
Single-molecule localization microscopy super-resolution methods rely on stochastic
blinking/binding events, which often occur multiple times from each emitter over the course …

Quantitative Analysis of Protein–Protein Equilibrium Constants in Cellular Environments Using Single-Molecule Localization Microscopy

LF Marcano-García, C Zaza, OPL Dalby… - Nano Letters, 2024 - ACS Publications
Current methods for determining equilibrium constants often operate in three-dimensional
environments, which may not accurately reflect interactions with membrane-bound proteins …

Nonblinking core–multishell InP/ZnSe/ZnS quantum dot bioconjugates for super-resolution imaging

L Zhou, B Yu, L Huang, H Cao, D Lin… - ACS Applied Nano …, 2022 - ACS Publications
Colloidal quantum dots (QDs) are vital fluorescent probes with several optical features
superior to those found in organic dyes for microscopy imaging. However, the emission …

Exploring fetal brain tumor glioblastoma symptom verification with self organizing maps and vulnerability data analysis

SK Natarajan, JS, SK Mathivanan, H Rajadurai… - Scientific Reports, 2024 - nature.com
Brain tumor glioblastoma is a disease that is caused for a child who has abnormal cells in
the brain, which is found using MRI “Magnetic Resonance Imaging” brain image using a …