Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications
H Wei, J Mo, L Tao, RJ Russell, AA Tymiak, G Chen… - Drug discovery today, 2014 - Elsevier
Highlights•Hydrogen/deuterium exchange mass spectrometry (HDX-MS) technology for
assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS …
assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS …
Protein chemistry combined with mass spectrometry for protein structure determination
The advent of soft-ionization mass spectrometry for biomolecules has opened up new
possibilities for the structural analysis of proteins. Combining protein chemistry methods with …
possibilities for the structural analysis of proteins. Combining protein chemistry methods with …
Contemporary hydrogen deuterium exchange mass spectrometry
Hydrogen/deuterium exchange (HDX) mass spectrometry (MS) emerged as a tool for
biochemistry and structural biology around 25 years ago. It has since become a key …
biochemistry and structural biology around 25 years ago. It has since become a key …
Protein hydrogen exchange measured at single-residue resolution by electron transfer dissociation mass spectrometry
Because of unparalleled sensitivity and tolerance to protein size, mass spectrometry (MS)
has become a popular method for measuring the solution hydrogen (1H/2H) exchange (HX) …
has become a popular method for measuring the solution hydrogen (1H/2H) exchange (HX) …
Hydrogen/deuterium exchange mass spectrometry with top-down electron capture dissociation for characterizing structural transitions of a 17 kDa protein
Amide H/D exchange (HDX) mass spectrometry (MS) is widely used for protein structural
studies. Traditionally, this technique involves protein labeling in D2O, followed by acid …
studies. Traditionally, this technique involves protein labeling in D2O, followed by acid …
MSTools—Web based application for visualization and presentation of HXMS data
D Kavan, P Man - International journal of mass spectrometry, 2011 - Elsevier
Hydrogen/deuterium exchange coupled to mass spectrometry is an excellent technique to
study protein structure changes and interactions, especially in cases where traditional high …
study protein structure changes and interactions, especially in cases where traditional high …
Top-down mass spectrometry: recent developments, applications and perspectives
Top-down mass spectrometry is an emerging approach for the analysis of intact proteins.
The term was coined as a contrast with the better-established, bottom-up strategy for …
The term was coined as a contrast with the better-established, bottom-up strategy for …
Radical solutions: Principles and application of electron‐based dissociation in mass spectrometry‐based analysis of protein structure
In recent years, electron capture (ECD) and electron transfer dissociation (ETD) have
emerged as two of the most useful methods in mass spectrometry‐based protein analysis …
emerged as two of the most useful methods in mass spectrometry‐based protein analysis …
Protein hydrogen exchange at residue resolution by proteolytic fragmentation mass spectrometry analysis
Hydrogen exchange technology provides a uniquely powerful instrument for measuring
protein structural and biophysical properties, quantitatively and in a nonperturbing way, and …
protein structural and biophysical properties, quantitatively and in a nonperturbing way, and …
Electron transfer dissociation facilitates the measurement of deuterium incorporation into selectively labeled peptides with single residue resolution
Mass spectrometry is routinely applied to measure the incorporation of deuterium into
proteins and peptides. The exchange of labile, heteroatom-bound hydrogens is mainly used …
proteins and peptides. The exchange of labile, heteroatom-bound hydrogens is mainly used …