The optical microscope has revolutionized biology since at least the 17th Century. Since
then, it has progressed from a largely observational tool to a powerful bioanalytical platform …

Synapses are highly specialized neuronal structures that are essential for
neurotransmission, and they are dynamically regulated throughout the lifetime. Although …

While a number of methods exist to investigate CRISPR off-target (OT) editing, few have
been compared head-to-head in primary cells after clinically relevant editing processes …

Protein labeling approaches are important to study proteins in living cells, and genome
editing tools make it possible to tag endogenous proteins to address the concerns …

The postsynaptic density (PSD) of excitatory synapses is built from a wide variety of
scaffolding proteins, receptors, and signaling molecules that collectively orchestrate synaptic …

Zebrafish provide an excellent model for in vivo cell biology studies because of their
amenability to live imaging. Protein visualization in zebrafish has traditionally relied on …

One of the main drivers of autism spectrum disorder is risk alleles within hundreds of genes,
which may interact within shared but unknown protein complexes. Here we develop a …

Integrin-mediated cell attachment rapidly induces tyrosine kinase signaling. Despite years of
research, the role of this signaling in integrin activation and focal adhesion assembly is …

Gene replacement therapies primarily rely on adeno-associated virus (AAV) vectors for
transgene expression. However, episomal expression can decline over time due to vector …

Abstract CRISPR/Cas9-mediated knock-in methods enable the labeling of individual
endogenous proteins to faithfully determine their spatiotemporal distribution in cells …