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Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Evaluating cell metabolism through autofluorescence imaging of NAD (P) H and FAD
Significance: Optical imaging using the endogenous fluorescence of metabolic cofactors has
enabled nondestructive examination of dynamic changes in cell and tissue function both in …
enabled nondestructive examination of dynamic changes in cell and tissue function both in …
[HTML][HTML] Defining epidermal basal cell states during skin homeostasis and wound healing using single-cell transcriptomics
Our knowledge of transcriptional heterogeneities in epithelial stem and progenitor cell
compartments is limited. Epidermal basal cells sustain cutaneous tissue maintenance and …
compartments is limited. Epidermal basal cells sustain cutaneous tissue maintenance and …
[HTML][HTML] MYC disrupts the circadian clock and metabolism in cancer cells
The MYC oncogene encodes MYC, a transcription factor that binds the genome through
sites termed E-boxes (5′-CACGTG-3′), which are identical to the binding sites of the …
sites termed E-boxes (5′-CACGTG-3′), which are identical to the binding sites of the …
[HTML][HTML] Aged stem cells reprogram their daily rhythmic functions to adapt to stress
Normal homeostatic functions of adult stem cells have rhythmic daily oscillations that are
believed to become arrhythmic during aging. Unexpectedly, we find that aged mice remain …
believed to become arrhythmic during aging. Unexpectedly, we find that aged mice remain …
Circadian rhythms in mitochondrial respiration
P De Goede, J Wefers, EC Brombacher… - Journal of …, 2018 - jme.bioscientifica.com
Many physiological processes are regulated with a 24-h periodicity to anticipate the
environmental changes of daytime to nighttime and vice versa. These 24-h regulations …
environmental changes of daytime to nighttime and vice versa. These 24-h regulations …
The epidermal circadian clock integrates and subverts brain signals to guarantee skin homeostasis
In mammals, the circadian clock network drives daily rhythms of tissue-specific homeostasis.
To dissect daily inter-tissue communication, we constructed a mouse minimal clock network …
To dissect daily inter-tissue communication, we constructed a mouse minimal clock network …
tauFisher predicts circadian time from a single sample of bulk and single-cell pseudobulk transcriptomic data
As the circadian clock regulates fundamental biological processes, disrupted clocks are
often observed in patients and diseased tissues. Determining the circadian time of the …
often observed in patients and diseased tissues. Determining the circadian time of the …
The MYCN protein in health and disease
MYCN is a member of the MYC family of proto-oncogenes. It encodes a transcription factor,
MYCN, involved in the control of fundamental processes during embryonal development …
MYCN, involved in the control of fundamental processes during embryonal development …
BMAL1-driven tissue clocks respond independently to light to maintain homeostasis
Circadian rhythms control organismal physiology throughout the day. At the cellular level,
clock regulation is established by a self-sustained Bmal1-dependent transcriptional …
clock regulation is established by a self-sustained Bmal1-dependent transcriptional …