CRISPR–Cas9 gRNA efficiency prediction: an overview of predictive tools and the role of deep learning

V Konstantakos, A Nentidis, A Krithara… - Nucleic Acids …, 2022 - academic.oup.com
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated
protein 9 (Cas9) system has become a successful and promising technology for gene …

[HTML][HTML] Latest developed strategies to minimize the off-target effects in CRISPR-Cas-mediated genome editing

M Naeem, S Majeed, MZ Hoque, I Ahmad - Cells, 2020 - mdpi.com
Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …

Plant breeding advancements with “CRISPR-Cas” genome editing technologies will assist future food security

M Ahmad - Frontiers in plant science, 2023 - frontiersin.org
Genome editing techniques are being used to modify plant breeding, which might increase
food production sustainably by 2050. A product made feasible by genome editing is …

Applications and challenges of CRISPR-Cas gene-editing to disease treatment in clinics

W Liu, L Li, J Jiang, M Wu, P Lin - Precision clinical medicine, 2021 - academic.oup.com
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated systems
(Cas) are efficient tools for targeting specific genes for laboratory research, agricultural …

CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer

M Vaghari-Tabari, P Hassanpour… - Cellular & Molecular …, 2022 - Springer
The CRISPR/Cas9 system is an RNA-based adaptive immune system in bacteria and
archaea. Various studies have shown that it is possible to target a wide range of human …

Current bioinformatics tools to optimize CRISPR/Cas9 experiments to reduce off-target effects

M Naeem, OS Alkhnbashi - International Journal of Molecular Sciences, 2023 - mdpi.com
The CRISPR-Cas system has evolved into a cutting-edge technology that has transformed
the field of biological sciences through precise genetic manipulation. CRISPR/Cas9 …

Genome editing and beyond: what does it mean for the future of plant breeding?

T Van Vu, S Das, G Hensel, JY Kim - Planta, 2022 - Springer
Main conclusion Genome editing offers revolutionized solutions for plant breeding to sustain
food production to feed the world by 2050. Therefore, genome-edited products are …

CRISPR interference screens reveal growth–robustness tradeoffs in Synechocystis sp. PCC 6803 across growth conditions

R Miao, M Jahn, K Shabestary, G Peltier… - The Plant …, 2023 - academic.oup.com
Barcoded mutant libraries are a powerful tool for elucidating gene function in microbes,
particularly when screened in multiple growth conditions. Here, we screened a pooled …

Using traditional machine learning and deep learning methods for on-and off-target prediction in CRISPR/Cas9: a review

Z Sherkatghanad, M Abdar, J Charlier… - Briefings in …, 2023 - academic.oup.com
Abstract CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats and
CRISPR-associated protein 9) is a popular and effective two-component technology used for …

[HTML][HTML] CRISPR-Cas9 system for plant genome editing: current approaches and emerging developments

JAV Montecillo, LL Chu, H Bae - Agronomy, 2020 - mdpi.com
Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic
engineering tool in various biological systems. This editing technology has been in the …