Unveiling an indole alkaloid diketopiperazine biosynthetic pathway that features a unique stereoisomerase and multifunctional methyltransferase

G Deletti, SD Green, C Weber, KN Patterson… - Nature …, 2023 - nature.com
Abstract The 2, 5-diketopiperazines are a prominent class of bioactive molecules. The
nocardioazines are actinomycete natural products that feature a pyrroloindoline …

Production of Diketopiperazine Derivatives by Pathway Engineering with Different Cyclodipeptide Synthases from Various Streptomyces Strains

SA Stierle, L Harken, SM Li - ACS Synthetic Biology, 2023 - ACS Publications
Cyclodipeptides from fungi and bacteria are often modified by different tailoring enzymes.
They display various biological and pharmacological activities, and some derivatives are …

A nucleobase-driven P450 peroxidase system enables regio-and stereo-specific formation of C─ C and C─ N bonds

G Wei, B Duan, TP Zhou, W Tian, C Sun, Z Lin… - Proceedings of the …, 2024 - pnas.org
P450 peroxidase activities are valued for their ability to catalyze complex chemical
transformations using economical H2O2; however, they have been largely underexplored …

Divergent Metabolism of Cyclo-l-Trp-l-Leu in Streptomyces albofaciens by Hydroxylation and Nucleobase Transfer with Two Cytochrome P450 Enzymes

Y Dai, D Ostendorff, SM Li - Journal of Natural Products, 2024 - ACS Publications
A three-gene salb cluster from Streptomyces albofaciens was proven to be responsible for
the formation of cyclo-l-Trp-l-Leu (cWL) derivatives. An Escherichia coli strain harboring the …

Intramolecular Coupling and Nucleobase Transfer – How Cytochrome P450 Enzymes GymBx Establish Their Chemoselectivity

J Freytag, A Martin, JC Müller, T Kelm, T Stehle… - …, 2024 - Wiley Online Library
Cyclodipeptides (CDPs) build an important class of secondary metabolites with a plethora of
pharmacological impact such as antibiotic, cytotoxic, antifungal properties or even as anti …

P450 in C–C coupling of cyclodipeptides with nucleobases

SA Stierle, L Harken, SM Li - Methods in Enzymology, 2023 - books.google.com
Bacterial cytochrome P450 enzymes catalyze various and often intriguing tailoring reactions
during the biosynthesis of natural products. In contrast to the majority of membrane-bound …