Advances in microfluidic strategies for single-cell research

Y Liu, Z Fan, L Qiao, B Liu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
A core challenge in biomedical and life science research is to decipher cell-to-cell
differences under pathological progression or external perturbation. Microfluidics-based …

A review of single-cell adhesion force kinetics and applications

A Shinde, K Illath, P Gupta, P Shinde, KT Lim, M Nagai… - Cells, 2021 - mdpi.com
Cells exert, sense, and respond to the different physical forces through diverse mechanisms
and translating them into biochemical signals. The adhesion of cells is crucial in various …

Vapor nanobubble-mediated photoporation constitutes a versatile intracellular delivery technology

J Ramon, R **ong, SC De Smedt, K Raemdonck… - Current Opinion in …, 2021 - Elsevier
Vapor nanobubble-mediated photoporation has evolved into a promising physical
intracellular delivery technology. When irradiated with short but intense laser pulses …

Metallic micro-ring device for highly efficient large cargo delivery in mammalian cells using infrared light pulses

A Shinde, P Shinde, S Kar, K Illath, S Dey… - Lab on a Chip, 2023 - pubs.rsc.org
Uniform transfection of biomolecules into live cells with high delivery efficiency and cell
viability is an immensely important area of biological research and has many biomedical …

Pulsed laser assisted high-throughput intracellular delivery in hanging drop based three dimensional cancer spheroids

P Gupta, S Kar, A Kumar, FG Tseng, S Pradhan… - Analyst, 2021 - pubs.rsc.org
Targeted intracellular delivery of biomolecules and therapeutic cargo enables the controlled
manipulation of cellular processes. Laser-based optoporation has emerged as a versatile …

Ultra-low intensity light pulses for large cargo delivery into hard-to-transfect cells using an rGO mixed PDMS microtip device

HH Padma, K Illath, D Dominic, HY Chang, M Nagai… - Lab on a Chip, 2024 - pubs.rsc.org
Nanoparticle-mediated photoporation has arisen as a universal intracellular delivery tool;
however, the direct interaction of nanoparticles and cells hampers its clinical translation …

Combinatorial physical methods for cellular therapy: towards the future of cellular analysis?

P Chakrabarty, K Illath, S Kar, M Nagai… - Journal of Controlled …, 2023 - Elsevier
The physical energy activated techniques for cellular delivery and analysis is one of the
most rapidly expanding research areas for a variety of biological and biomedical …

Nanomaterial-mediated photoporation for intracellular delivery

L Wang, X Wei, H Liu, Y Fan - Acta Biomaterialia, 2023 - Elsevier
Translocation of extrinsic molecules into living cells is becoming increasingly crucial in
biological studies ranging from cell engineering to biomedical applications. The concerns …

Microfluidic based physical approaches towards single-cell intracellular delivery and analysis

K Kaladharan, A Kumar, P Gupta, K Illath, TS Santra… - Micromachines, 2021 - mdpi.com
The ability to deliver foreign molecules into a single living cell with high transfection
efficiency and high cell viability is of great interest in cell biology for applications in …

Ultrathin SU-8 membrane for highly efficient tunable cell patterning and massively parallel large biomolecular delivery

P Shinde, A Shinde, S Kar, K Illath, M Nagai, FG Tseng… - Lab on a Chip, 2023 - pubs.rsc.org
Cell patterning is a powerful technique for the precise control and arrangement of cells,
enabling detailed single-cell analysis with broad applications in therapeutics, diagnostics …