[HTML][HTML] Anything but ordinary–emerging splicing mechanisms in eukaryotic gene regulation

NH Gehring, JY Roignant - Trends in Genetics, 2021 - cell.com
Splicing of precursor mRNAs (pre-mRNA) is an important step during eukaryotic gene
expression. The identification of the actual splice sites and the proper removal of introns are …

Cryo-EM snapshots of the human spliceosome reveal structural adaptions for splicing regulation

SM Fica - Current opinion in structural biology, 2020 - Elsevier
Highlights•Cryo-EM structures reveal a more complex proteome for the human spliceosome
compared to budding yeast.•Movements of three loop-like elements of the core protein Prp8 …

Retained introns in long RNA-seq reads are not reliably detected in sample-matched short reads

JK David, SK Maden, MA Wood, RF Thompson… - Genome biology, 2022 - Springer
Background There is growing interest in retained introns in a variety of disease contexts
including cancer and aging. Many software tools have been developed to detect retained …

Transcription and splicing dynamics during early Drosophila development

P Prudêncio, R Savisaar, K Rebelo, RG Martinho… - Rna, 2022 - rnajournal.cshlp.org
Widespread cotranscriptional splicing has been demonstrated from yeast to human.
However, most studies to date addressing the kinetics of splicing relative to transcription …

Two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

MT Parker, K Knop, GJ Barton, GG Simpson - bioRxiv, 2020 - biorxiv.org
Transcription of eukaryotic genomes involves complex alternative processing of RNAs.
Sequencing of full-length RNAs using long-reads reveals the true complexity of processing …

Proofreading and Progression at the Catalytic Stages of Splicing: Novel Insights into the Roles and Regulation of Deah-Box ATPases

CAJ Craddock - 2022 - search.proquest.com
Pre-mRNA splicing is an essential step in eukaryotic gene expression, wherein interrupting
sequences (“introns”) are excised from newly transcribed precursor messenger RNA (pre …