1. INTRODUCTION The design of organically modified surfaces is a rapidly expanding field
of research in materials science in which the central purpose is access to materials …

This article reviews the development of immobilized-metal affinity chromatography (IMAC)
and describes its most important applications. We provide an overview on the use of IMAC in …

Recombinant enzymes have gained prominence due to their diverse functionalities and
specificity and are often a greener alternative in biocatalysis. This context makes purifying …

Mass spectrometry (MS)-based phosphoproteomics has achieved extraordinary success in
qualitative and quantitative analysis of cellular protein phosphorylation. Considering that an …

Mass spectrometry (MS)-based proteomics has become the preferred tool for the analysis of
protein phosphorylation. To be successful at such an endeavor, there is a requirement for an …

More than 300 different types of protein post‐translational modifications (PTMs) have been
described, many of which are known to have pivotal roles in cellular physiology and …

Functionalized magnetic materials (FMMs) have been widely used in analytical chemistry.
For sample preparation, FMMs show many advantages including easy surface modification …

After 35 years of development, immobilized metal ion affinity chromatography (IMAC) has
evolved into a popular protein purification technique. This review starts with a discussion of …

Phosphorylation is a form of protein posttranslational modification (PTM) that regulates many
biological processes. Whereas phosphoproteomics is a scientific discipline that identifies …

Reversible protein phosphorylation is a key event in numerous biological processes. Mass
spectrometry (MS) is the most powerful analysis tool in modern phosphoproteomics …