While the primary sequences of human proteins have been cataloged for over a decade,
determining how these are organized into a dynamic collection of multiprotein assemblies …

Proteins are responsible for most intracellular functions, which they perform as part of higher-
order molecular complexes, located within defined subcellular niches. Localization is both …

Classical G-protein-coupled receptor (GPCR) signaling takes place in response to
extracellular stimuli and involves receptors and heterotrimeric G proteins located at the …

High-throughput dynamic imaging of cells and organelles is essential for understanding
complex cellular responses. We report Mantis, a high-throughput 4D microscope that …

Imbalances in lipid storage and secretion lead to the accumulation of hepatocyte lipid
droplets (LDs)(ie, hepatic steatosis). Our understanding of the mechanisms that govern the …

Membrane trafficking, a fundamental cellular process encompassing the transport of
molecules to specific organelles, endocytosis at the plasma membrane and protein …

The eukaryotic microrchidia (MORC) protein family are DNA g yrase, H sp90, histidine k
inase, Mut L (GHKL)-type ATPases involved in gene expression regulation and chromatin …

Soft X-ray tomography (SXT) is an invaluable tool for quantitatively analyzing cellular
structures at sub-optical isotropic resolution. However, it has traditionally depended on …

The success of artificial intelligence (AI) algorithms in predicting protein structure and more
recently, protein interactions, demonstrates the power and potential of machine learning and …

Cryo-electron tomography (cryoET) has emerged as a powerful structural biology tool for
understanding protein complexes in their native cellular environments. Presently, 3D …