Komagataella phaffii as Emerging Model Organism in Fundamental Research

L Bernauer, A Radkohl, LGK Lehmayer… - Frontiers in …, 2021‏ - frontiersin.org
Komagataella phaffii (Pichia pastoris) is one of the most extensively applied yeast species in
pharmaceutical and biotechnological industries, and, therefore, also called the biotech …

[HTML][HTML] Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

C Bustos, J Quezada, R Veas, C Altamirano… - Metabolites, 2022‏ - mdpi.com
Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly
important microorganism for recombinant protein production. This yeast species has gained …

OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression

K Claes, D Van Herpe, R Vanluchene, C Roels… - Nature …, 2024‏ - nature.com
The industrial yeast Komagataella phaffii (formerly named Pichia pastoris) is commonly
used to synthesize recombinant proteins, many of which are used as human therapeutics or …

[HTML][HTML] Going beyond the limit: Increasing global translation activity leads to increased productivity of recombinant secreted proteins in Pichia pastoris

J Staudacher, C Rebnegger, T Dohnal, N Landes… - Metabolic …, 2022‏ - Elsevier
Yeasts are widely used cell factories for commercial heterologous protein production,
however, specific productivities are usually tightly coupled to biomass formation. This greatly …

[HTML][HTML] Functional and mass spectrometric evaluation of an anti-tick antigen based on the P0 peptide conjugated to Bm86 protein

A Rodríguez Mallón, L Javier González… - Pathogens, 2020‏ - mdpi.com
A synthetic 20 amino acid peptide of the ribosomal protein P0 from ticks, when conjugated to
keyhole limpet hemocyanin from Megathura crenulata and used as an immunogen against …

Identification of genetic variants of the industrial yeast Komagataella phaffii (Pichia pastoris) that contribute to increased yields of secreted heterologous proteins

B Offei, S Braun-Galleani, A Venkatesh, WT Casey… - PLoS …, 2022‏ - journals.plos.org
The yeast Komagataella phaffii (formerly called Pichia pastoris) is used widely as a host for
secretion of heterologous proteins, but only a few isolates of this species exist and all the …

Genotypic and phenotypic diversity among Komagataella species reveals a hidden pathway for xylose utilization

L Heistinger, JC Dohm, BG Paes, D Koizar… - Microbial Cell …, 2022‏ - Springer
Abstract Background The yeast genus Komagataella currently consists of seven
methylotrophic species isolated from tree environments. Well-characterized strains of K …

The Degree and Length of O‐Glycosylation of Recombinant Proteins Produced in Pichia pastoris Depends on the Nature of the Protein and the Process Type

B Radoman, C Grünwald‐Gruber… - Biotechnology …, 2021‏ - Wiley Online Library
The methylotrophic yeast Pichia pastoris is known as an efficient host for the production of
heterologous proteins. While N‐linked protein glycosylation is well characterized in P …

Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltii

F Dutreux, A Dutta, E Peltier, S Bibi-Triki… - PLoS …, 2023‏ - journals.plos.org
Meiotic recombination is a driving force for genome evolution, deeply characterized in a few
model species, notably in the budding yeast Saccharomyces cerevisiae. Interestingly, Zip2 …

[HTML][HTML] Heterologous Expression of Extracellular Proteinase pAsPs of Aspergillus pseudotamarii in Komagataella phaffii

AV Zadorozhny, ME Voskoboev, DV Bochkov… - International Journal of …, 2022‏ - mdpi.com
Neutral protease pAsPs gene was obtained by sequence optimization of NpI protease from
Aspergillus pseudotamarii. pAsPs was for the first time integrated in the genome of yeast …