The production of ethanol by yeast fermentation represents the largest of all global
biotechnologies. Consequently, the yeast Saccharomyces cerevisiae is the world's premier …

Wine is an archetypal traditional fermented beverage with strong territorial and socio-cultural
connotations. Its 7000 year history is patterned by a tradition of innovation. Every value …

The Sc2. 0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2. 0 chromosomes assembled. Here, we describe …

We describe construction of the synthetic yeast chromosome XI (synXI) and reveal the
effects of redesign at non-coding DNA elements. The 660-kb synthetic yeast genome project …

Abstract Synthetic Chromosome Rearrangement and Modification by LoxP-mediated
Evolution (SCRaMbLE) is a promising tool to study genomic rearrangements. However, the …

Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels.
However, product toxicity has hindered improvements in biofuel production. Here, we …

Conventional plasmid-based gene expression tends to introduce genetic instability and
gene copy number variations that lead to degenerated production. The limited number of …

Highlights•The lack of predictive modeling limits biosystems design for biofuel production.•
Automation in build and test accelerates strain engineering for biofuel research.•Microplates …

Aneuploidy compromises genomic stability, often leading to embryo inviability, and is
frequently associated with tumorigenesis and aging. Different aneuploid chromosome …

Synthetic biology, genome engineering and directed evolution offer innumerable tools to
expedite engineering of strains for optimising biosynthetic pathways. One of the most radical …