Next-generation lineage tracing and fate map** to interrogate development

S VanHorn, SA Morris - Developmental cell, 2021‏ - cell.com
Lineage tracing and fate map**, overlap** yet distinct disciplines to follow cells and
their progeny, have evolved rapidly over the last century. Lineage tracing aims to identify all …

Cellular dynamics of EMT: lessons from live in vivo imaging of embryonic development

JD Amack - Cell Communication and Signaling, 2021‏ - Springer
Epithelial-mesenchymal transition (EMT) refers to a process in which epithelial cells lose
apical-basal polarity and loosen cell–cell junctions to take on mesenchymal cell …

Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale

J Wu, Z Lu, D Jiang, Y Guo, H Qiao, Y Zhang, T Zhu… - Cell, 2021‏ - cell.com
Long-term subcellular intravital imaging in mammals is vital to study diverse intercellular
behaviors and organelle functions during native physiological processes. However, optical …

Long-term intravital subcellular imaging with confocal scanning light-field microscopy

Z Lu, S Zuo, M Shi, J Fan, J ** embryos
J Stegmaier, F Amat, WC Lemon, K McDole, Y Wan… - Developmental cell, 2016‏ - cell.com
We present the Real-time Accurate Cell-shape Extractor (RACE), a high-throughput image
analysis framework for automated three-dimensional cell segmentation in large-scale …

Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination

S Takanezawa, T Saitou, T Imamura - Nature Communications, 2021‏ - nature.com
Two-photon excitation can lower phototoxicity and improve penetration depth, but its narrow
excitation range restricts its applications in light-sheet microscopy. Here, we propose simple …

Intravital imaging of mouse embryos

Q Huang, MA Cohen, FC Alsina, G Devlin, A Garrett… - Science, 2020‏ - science.org
Embryonic development is a complex process that is unamenable to direct observation. In
this study, we implanted a window to the mouse uterus to visualize the develo** embryo …

Efficient processing and analysis of large-scale light-sheet microscopy data

F Amat, B Höckendorf, Y Wan, WC Lemon, K McDole… - Nature protocols, 2015‏ - nature.com
Light-sheet microscopy is a powerful method for imaging the development and function of
complex biological systems at high spatiotemporal resolution and over long time scales …