Advances and prospects of Bacillus subtilis cellular factories: from rational design to industrial applications

Y Gu, X Xu, Y Wu, T Niu, Y Liu, J Li, G Du, L Liu - Metabolic engineering, 2018 - Elsevier
Bacillus subtilis is the most characterized gram-positive bacterium that has significant
attributes, such as growing well on cheap carbon sources, possessing clear inherited …

Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond

W Cui, L Han, F Suo, Z Liu, L Zhou, Z Zhou - World Journal of Microbiology …, 2018 - Springer
Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low% G+
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …

Pyruvate-responsive genetic circuits for dynamic control of central metabolism

X Xu, X Li, Y Liu, Y Zhu, J Li, G Du, J Chen… - Nature Chemical …, 2020 - nature.com
Dynamic regulation is a promising strategy for fine-tuning metabolic fluxes in microbial cell
factories. However, few of these synthetic regulatory systems have been developed for …

Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots

Y Cao, Z Zhang, N Ling, Y Yuan, X Zheng… - Biology and fertility of …, 2011 - Springer
Fusarium wilt is one of the major constraints on cucumber production worldwide. Several
strategies have been used to control the causative pathogen, Fusarium oxysporum f. sp …

Simple, fast and high‐efficiency transformation system for directed evolution of cellulase in Bacillus subtilis

XZ Zhang, YHP Zhang - Microbial biotechnology, 2011 - Wiley Online Library
Bacillus subtilis can serve as a powerful platform for directed evolution, especially for
secretory enzymes. However, cloning and transformation of a DNA mutant library in B …

Production of specific-molecular-weight hyaluronan by metabolically engineered Bacillus subtilis 168

P **, Z Kang, P Yuan, G Du, J Chen - Metabolic engineering, 2016 - Elsevier
Low-molecular-weight hyaluronan (LMW-HA) has attracted much attention because of its
many potential applications. Here, we efficiently produced specific LMW-HAs from sucrose …

An overview and future prospects of recombinant protein production in Bacillus subtilis

H Yang, J Qu, W Zou, W Shen, X Chen - Applied Microbiology and …, 2021 - Springer
Bacillus subtilis is a well-characterized Gram-positive bacterium and a valuable host for
recombinant protein production because of its efficient secretion ability, high yield, and non …

Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis

X Yan, HJ Yu, Q Hong, SP Li - Applied and Environmental …, 2008 - journals.asm.org
We have developed a fast and accurate method to engineer the Bacillus subtilis genome
that involves fusing by PCR two flanking homology regions with an antibiotic resistance …

Modular pathway engineering of Bacillus subtilis for improved N-acetylglucosamine production

Y Liu, Y Zhu, J Li, H Shin, RR Chen, G Du, L Liu… - Metabolic …, 2014 - Elsevier
In previous work, we constructed a recombinant Bacillus subtilis strain for microbial
production of N-acetylglucosamine (GlcNAc), which has applications in nutraceuticals and …

Characterization and application of endogenous phase-dependent promoters in Bacillus subtilis

S Yang, G Du, J Chen, Z Kang - Applied microbiology and biotechnology, 2017 - Springer
Bacillus subtilis as an important host has been widely used in synthetic biology, metabolic
engineering, and production of industrial enzymes. To fully take advantage of this organism …