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Advances and prospects of Bacillus subtilis cellular factories: from rational design to industrial applications
Bacillus subtilis is the most characterized gram-positive bacterium that has significant
attributes, such as growing well on cheap carbon sources, possessing clear inherited …
attributes, such as growing well on cheap carbon sources, possessing clear inherited …
Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond
W Cui, L Han, F Suo, Z Liu, L Zhou, Z Zhou - World Journal of Microbiology …, 2018 - Springer
Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low% G+
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …
Pyruvate-responsive genetic circuits for dynamic control of central metabolism
Dynamic regulation is a promising strategy for fine-tuning metabolic fluxes in microbial cell
factories. However, few of these synthetic regulatory systems have been developed for …
factories. However, few of these synthetic regulatory systems have been developed for …
Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots
Fusarium wilt is one of the major constraints on cucumber production worldwide. Several
strategies have been used to control the causative pathogen, Fusarium oxysporum f. sp …
strategies have been used to control the causative pathogen, Fusarium oxysporum f. sp …
Simple, fast and high‐efficiency transformation system for directed evolution of cellulase in Bacillus subtilis
XZ Zhang, YHP Zhang - Microbial biotechnology, 2011 - Wiley Online Library
Bacillus subtilis can serve as a powerful platform for directed evolution, especially for
secretory enzymes. However, cloning and transformation of a DNA mutant library in B …
secretory enzymes. However, cloning and transformation of a DNA mutant library in B …
Production of specific-molecular-weight hyaluronan by metabolically engineered Bacillus subtilis 168
P **, Z Kang, P Yuan, G Du, J Chen - Metabolic engineering, 2016 - Elsevier
Low-molecular-weight hyaluronan (LMW-HA) has attracted much attention because of its
many potential applications. Here, we efficiently produced specific LMW-HAs from sucrose …
many potential applications. Here, we efficiently produced specific LMW-HAs from sucrose …
An overview and future prospects of recombinant protein production in Bacillus subtilis
H Yang, J Qu, W Zou, W Shen, X Chen - Applied Microbiology and …, 2021 - Springer
Bacillus subtilis is a well-characterized Gram-positive bacterium and a valuable host for
recombinant protein production because of its efficient secretion ability, high yield, and non …
recombinant protein production because of its efficient secretion ability, high yield, and non …
Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis
X Yan, HJ Yu, Q Hong, SP Li - Applied and Environmental …, 2008 - journals.asm.org
We have developed a fast and accurate method to engineer the Bacillus subtilis genome
that involves fusing by PCR two flanking homology regions with an antibiotic resistance …
that involves fusing by PCR two flanking homology regions with an antibiotic resistance …
Modular pathway engineering of Bacillus subtilis for improved N-acetylglucosamine production
In previous work, we constructed a recombinant Bacillus subtilis strain for microbial
production of N-acetylglucosamine (GlcNAc), which has applications in nutraceuticals and …
production of N-acetylglucosamine (GlcNAc), which has applications in nutraceuticals and …
Characterization and application of endogenous phase-dependent promoters in Bacillus subtilis
S Yang, G Du, J Chen, Z Kang - Applied microbiology and biotechnology, 2017 - Springer
Bacillus subtilis as an important host has been widely used in synthetic biology, metabolic
engineering, and production of industrial enzymes. To fully take advantage of this organism …
engineering, and production of industrial enzymes. To fully take advantage of this organism …