Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that …

The glycoproteome remains severely understudied because of significant analytical
challenges associated with glycoproteomics, the system-wide analysis of intact …

Background The gut microbiota has been shown to be closely associated with human health
and disease. While next-generation sequencing can be readily used to profile the microbiota …

Background N-linked glycoprotein is a highly interesting class of proteins for clinical and
biological research. The large-scale characterization of N-linked glycoproteins …

De novo uridine-diphosphate-N-acetylglucosamine (UDP-GlcNAc) biosynthesis requires
glucose, glutamine, acetyl-CoA and uridine, however GlcNAc salvaged from glycoconjugate …

Protein glycosylation plays a key role in various biological processes and disease-related
pathological progression. Mass spectrometry (MS)-based glycoproteomics is a powerful …

The development of methods to isolate and enrich low-abundance glycopeptides from
biological samples is crucial to glycoproteomics. Herein, we present an easy and one-step …

Sample pretreatment is a fundamental and essential step in almost all analytical procedures,
especially for the analysis of biological and environmental samples with complex matrix. In …

Over 17,000 papers related to proteomics have been published since our 2011− 2013
review. A subset are true gems reporting amazing applications of proteomics to better …

Rationale Accurate characterization of glycopeptides without a prior glycan cleavage could
provide valuable information on site‐specific glycosylation, which is critical to reveal the …