Everything you ever wanted to know about PKA regulation and its involvement in mammalian sperm capacitation

CB Graf, C Ritagliati, C Stival, GM Luque… - Molecular and Cellular …, 2020‏ - Elsevier
Abstract The 3′, 5′-cyclic adenosine monophosphate (cAMP) dependent protein kinase
(PKA) is a tetrameric holoenzyme comprising a set of two regulatory subunits (PKA-R) and …

Biosensors with metal ion–phosphate chelation interaction for molecular recognition

X Ma, Y Hao, X Dong, N **a - Molecules, 2023‏ - mdpi.com
Biosensors show promising prospects in the assays of various targets due to their
advantages of high sensitivity, good selectivity and rapid response. Molecular recognition is …

A dual-potential electrochemiluminescence ratiometric approach based on graphene quantum dots and luminol for highly sensitive detection of protein kinase activity

HF Zhao, RP Liang, JW Wang, JD Qiu - Chemical Communications, 2015‏ - pubs.rsc.org
A novel Au NP mediated dual-potential ECL ratiometric approach for highly sensitive protein
kinase activity and inhibition assay has been developed based on the simultaneous …

Electrochemically mediated polymerization for highly sensitive detection of protein kinase activity

Q Hu, Q Wang, C Jiang, J Zhang, J Kong… - Biosensors and …, 2018‏ - Elsevier
Protein kinases play a pivotal role in cellular regulation and signal transduction, the
detection of protein kinase activity and inhibition is therefore of great importance to clinical …

Phosphorylation-directed assembly of a single quantum dot based nanosensor for protein kinase assay

L Wang, Y Yang, C Zhang - Analytical Chemistry, 2015‏ - ACS Publications
Protein kinases play crucial roles in intracellular signal transduction and metabolic
pathways, and the monitoring of protein kinase activity is essential to the understanding of …

Label-free fluorescence assay for protein kinase based on peptide biomineralized gold nanoclusters as signal sensing probe

W Song, Y Wang, RP Liang, L Zhang, JD Qiu - Biosensors and …, 2015‏ - Elsevier
A label-free, sensitive and simple method to detect protein kinase based on the selective
aggregation of phosphorylated peptide-gold nanoclusters (peptide-AuNCs) triggered by Zr …

Aptamer based electrochemical assay for protein kinase activity by coupling hybridization chain reaction

LP Jia, RN Zhao, LJ Wang, RN Ma, W Zhang… - Biosensors and …, 2018‏ - Elsevier
The present work reported a simple, lable-free and sensitive electrochemical method for the
detection of protein kinase A (PKA) activity. This method was based on the specific …

Upconversion nanophosphor: an efficient phosphopeptides-recognizing matrix and luminescence resonance energy transfer donor for robust detection of protein …

C Liu, L Chang, H Wang, J Bai, W Ren, Z Li - Analytical chemistry, 2014‏ - ACS Publications
Protein kinases play important regulatory roles in intracellular signal transduction pathways.
The aberrant activities of protein kinases are closely associated with the development of …

Rare Earth Ion Mediated Fluorescence Accumulation on a Single Microbead: An Ultrasensitive Strategy for the Detection of Protein Kinase Activity at the Single‐Cell …

X Zhang, C Liu, H Wang, H Wang… - Angewandte Chemie …, 2015‏ - Wiley Online Library
A single microbead‐based fluorescence imaging (SBFI) strategy that enables detection of
protein kinase activity from single cell lysates is reported. We systematically investigated the …

An ultrasensitive flow cytometric immunoassay based on bead surface-initiated template-free DNA extension

L Zhu, D Chen, X Lu, Y Qi, P He, C Liu, Z Li - Chemical Science, 2018‏ - pubs.rsc.org
Proteins lack the duplication mechanism like nucleic acids, so the connection of
immunoassays with effective nucleic acid amplification techniques has become a powerful …