Structural biology of CRISPR–Cas immunity and genome editing enzymes
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …
a wide range of genome editing and biotechnological applications. In this Review, we …
Gaussian accelerated molecular dynamics: Principles and applications
Gaussian accelerated molecular dynamics (GaMD) is a robust computational method for
simultaneous unconstrained enhanced sampling and free energy calculations of …
simultaneous unconstrained enhanced sampling and free energy calculations of …
Functional DNA regulated CRISPR-Cas12a sensors for point-of-care diagnostics of non-nucleic-acid targets
Y ** allosteric communications within individual proteins
Allostery in proteins influences various biological processes such as regulation of gene
transcription and activities of enzymes and cell signaling. Computational approaches for …
transcription and activities of enzymes and cell signaling. Computational approaches for …
Clustered regularly interspaced short palindromic repeats/Cas9 triggered isothermal amplification for site-specific nucleic acid detection
M Huang, X Zhou, H Wang, D **ng - Analytical chemistry, 2018 - ACS Publications
A novel CRISPR/Cas9 triggered isothermal exponential amplification reaction (CAS-
EXPAR) strategy based on CRISPR/Cas9 cleavage and nicking endonuclease (NEase) …
EXPAR) strategy based on CRISPR/Cas9 cleavage and nicking endonuclease (NEase) …
New Insights into the Cooperativity and Dynamics of Dimeric Enzymes
KW Chen, TY Sun, YD Wu - Chemical Reviews, 2023 - ACS Publications
A survey of protein databases indicates that the majority of enzymes exist in oligomeric
forms, with about half of those found in the UniProt database being homodimeric …
forms, with about half of those found in the UniProt database being homodimeric …
Eigenvector centrality for characterization of protein allosteric pathways
Determining the principal energy-transfer pathways responsible for allosteric communication
in biomolecules remains challenging, partially due to the intrinsic complexity of the systems …
in biomolecules remains challenging, partially due to the intrinsic complexity of the systems …
Catalytic Mechanism of Non-Target DNA Cleavage in CRISPR-Cas9 Revealed by Ab Initio Molecular Dynamics
CRISPR-Cas9 is a cutting-edge genome editing technology, which uses the endonuclease
Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is …
Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is …