Single-particle cryo-electron microscopy (cryo-EM SPA) has recently emerged as an
exceptionally well-suited technique for determining the structure of membrane proteins …

Cryogenic electron microscopy (cryo-EM) is now one of the most powerful and widely used
methods to determine high-resolution structures of macromolecules. A major bottleneck of …

While advances in single-particle cryo-EM have enabled the structural determination of
macromolecular complexes at atomic resolution, particle orientation bias (the …

Ornithine transcarbamylase (OTC) plays a significant role in the urea cycle, a metabolic
pathway functioning in the liver to detoxify ammonia. OTC deficiency (OTCD) is the most …

The heterodimeric Rab3GAP complex is a guanine nucleotide exchange factor (GEF) for the
Rab18 GTPase that regulates lipid droplet metabolism, ER-to-Golgi trafficking, secretion …

Cryo-sample preparation is a vital step in the process of obtaining high-resolution structures
of macromolecules by using the single-particle cryo–electron microscopy (cryo-EM) method; …

Abstract Tumor-suppressor let-7 pre-microRNAs (miRNAs) are regulated by terminal
uridylyltransferases TUT7 and TUT4 that either promote let-7 maturation by adding a single …

Influenza virus genome encapsidation is essential for the formation of a helical viral
ribonucleoprotein (vRNP) complex composed of nucleoproteins (NP), the trimeric …

Sample preparation can present a significant hurdle within single particle cryo-electron
microscopy (cryoEM), resulting in issues with reproducibility, data quality or an inability to …

The Bunyavirales order is a large and diverse group of segmented negative-strand RNA
viruses. Several virus families within this order contain important human pathogens …