Simple, yet efficient conformational sampling methods for reproducing/predicting biologically rare events of proteins

R Harada - Bulletin of the Chemical Society of Japan, 2018 - academic.oup.com
The biological functions of proteins are strongly related to their conformational transitions. To
elucidate the essential dynamics, molecular dynamics (MD) simulation has become a …

The role of ATP in solubilizing RNA‐binding protein fused in sarcoma

H Aida, Y Shigeta, R Harada - Proteins: Structure, Function, and …, 2022 - Wiley Online Library
Intrinsically disordered protein (IDP) plays an important role in liquid–liquid phase
separation (LLPS). RNA‐binding protein fused in sarcoma (FUS) is a well‐studied IDP that …

PaCS-Toolkit: Optimized Software Utilities for Parallel Cascade Selection Molecular Dynamics (PaCS-MD) Simulations and Subsequent Analyses

S Ikizawa, T Hori, TN Wijaya, H Kono… - The Journal of …, 2024 - ACS Publications
Parallel cascade selection molecular dynamics (PaCS-MD) is an enhanced conformational
sampling method conducted as a “repetition of time leaps in parallel worlds”, comprising …

Protein–ligand dissociation simulated by parallel cascade selection molecular dynamics

DP Tran, K Takemura, K Kuwata… - Journal of chemical theory …, 2018 - ACS Publications
We investigated the dissociation process of tri-N-acetyl-d-glucosamine from hen egg white
lysozyme using parallel cascade selection molecular dynamics (PaCS-MD), which …

Determination of the Association between Mesotrione Sensitivity and Conformational Change of 4-Hydroxyphenylpyruvate Dioxygenase via Free-Energy Analyses

Y Munei, K Hengphasatporn, Y Hori… - Journal of Agricultural …, 2023 - ACS Publications
One widely known herbicide target is 4-hydroxyphenylpyruvate dioxygenase (HPPD). Avena
sativa HPPD is less sensitive to mesotrione (herbicide) than Arabidopsis thaliana HPPD …

Efficient conformational search based on structural dissimilarity sampling: applications for reproducing structural transitions of proteins

R Harada, Y Shigeta - Journal of Chemical Theory and …, 2017 - ACS Publications
Structural Dissimilarity Sampling (SDS) is proposed as an efficient conformational search
method to promote structural transitions essential for the biological functions of proteins. In …

Structural Validation by the G-Factor Properly Regulates Boost Potentials Imposed in Conformational Sampling of Proteins

T Yasuda, R Morita, Y Shigeta… - Journal of Chemical …, 2022 - ACS Publications
Free energy landscapes (FELs) of proteins are indispensable for evaluating thermodynamic
properties. Molecular dynamics (MD) simulation is a computational method for calculating …

Preferential Door for Ligand Binding and Unbinding Pathways in Inhibited Human Acetylcholinesterase

N Kongkaew, K Hengphasatporn… - The Journal of …, 2024 - ACS Publications
Rising global population and increased food demands have resulted in the increased use of
organophosphate pesticides (OPs), leading to toxin accumulation and transmission to …

Ligand binding path sampling based on parallel cascade selection molecular dynamics: LB-PaCS-MD

H Aida, Y Shigeta, R Harada - Materials, 2022 - mdpi.com
Parallel cascade selection molecular dynamics (PaCS-MD) is a rare-event sampling method
that generates transition pathways between a reactant and product. To sample the transition …

Hybrid Cascade-type molecular dynamics with a Markov state model for efficient free energy calculations

R Harada, Y Shigeta - Journal of Chemical Theory and …, 2018 - ACS Publications
A protocol for calculating free energy landscapes (FELs) is proposed based on a
combination of two cascade-type molecular dynamics (MD) methods, parallel cascade …