Computational tools to unmask transposable elements

P Goerner-Potvin, G Bourque - Nature Reviews Genetics, 2018 - nature.com
A substantial proportion of the genome of many species is derived from transposable
elements (TEs). Moreover, through various self-copying mechanisms, TEs continue to …

Principles and innovative technologies for decrypting noncoding RNAs: from discovery and functional prediction to clinical application

YM Sun, YQ Chen - Journal of hematology & oncology, 2020 - Springer
Noncoding RNAs (ncRNAs) are a large segment of the transcriptome that do not have
apparent protein-coding roles, but they have been verified to play important roles in diverse …

TEtranscripts: a package for including transposable elements in differential expression analysis of RNA-seq datasets

Y **, OH Tam, E Paniagua, M Hammell - Bioinformatics, 2015 - academic.oup.com
Motivation: Most RNA-seq data analysis software packages are not designed to handle the
complexities involved in properly apportioning short sequencing reads to highly repetitive …

piRNA-guided transposon cleavage initiates Zucchini-dependent, phased piRNA production

BW Han, W Wang, C Li, Z Weng, PD Zamore - Science, 2015 - science.org
PIWI-interacting RNAs (piRNAs) protect the animal germ line by silencing transposons.
Primary piRNAs, generated from transcripts of genomic transposon “junkyards”(piRNA …

Relaxed targeting rules help PIWI proteins silence transposons

I Gainetdinov, J Vega-Badillo, K Cecchini, A Bagci… - Nature, 2023 - nature.com
In eukaryotes, small RNA guides, such as small interfering RNAs and microRNAs, direct
AGO-clade Argonaute proteins to regulate gene expression and defend the genome against …

A single mechanism of biogenesis, initiated and directed by PIWI proteins, explains piRNA production in most animals

I Gainetdinov, C Colpan, A Arif, K Cecchini, PD Zamore - Molecular cell, 2018 - cell.com
In animals, PIWI-interacting RNAs (piRNAs) guide PIWI proteins to silence transposons and
regulate gene expression. The mechanisms for making piRNAs have been proposed to …

Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers

Y Fu, PH Wu, T Beane, PD Zamore, Z Weng - BMC genomics, 2018 - Springer
Background RNA-seq and small RNA-seq are powerful, quantitative tools to study gene
regulation and function. Common high-throughput sequencing methods rely on polymerase …

[HTML][HTML] Large Drosophila germline piRNA clusters are evolutionarily labile and dispensable for transposon regulation

D Gebert, LK Neubert, C Lloyd, J Gui, R Lehmann… - Molecular Cell, 2021 - cell.com
PIWI proteins and their guiding Piwi-interacting small RNAs (piRNAs) are crucial for fertility
and transposon defense in the animal germline. In most species, the majority of piRNAs are …

The evolutionarily conserved piRNA-producing locus pi6 is required for male mouse fertility

PH Wu, Y Fu, K Cecchini, DM Özata, A Arif, T Yu… - Nature …, 2020 - nature.com
Abstract Pachytene PIWI-interacting RNAs (piRNAs), which comprise> 80% of small RNAs
in the adult mouse testis, have been proposed to bind and regulate target RNAs like …

Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm

YH Sun, A Wang, C Song, G Shankar… - Nature …, 2021 - nature.com
Sperm contributes diverse RNAs to the zygote. While sperm small RNAs have been shown
to impact offspring phenotypes, our knowledge of the sperm transcriptome, especially the …