Illuminating entomological dark matter with DNA barcodes in an era of insect decline, deep learning, and genomics

R Meier, MKN Lawniczak… - Annual Review of …, 2024 - annualreviews.org
Most insects encountered in the field are initially entomological dark matter in that they
cannot be identified to species while alive. This explains the enduring quest for efficient …

Toward global integration of biodiversity big data: a harmonized metabarcode data generation module for terrestrial arthropods

P Arribas, C Andújar, K Bohmann, JR DeWaard… - …, 2022 - academic.oup.com
Metazoan metabarcoding is emerging as an essential strategy for inventorying biodiversity,
with diverse projects currently generating massive quantities of community-level data. The …

ONTbarcoder and MinION barcodes aid biodiversity discovery and identification by everyone, for everyone

A Srivathsan, L Lee, K Katoh, E Hartop, SN Kutty… - BMC biology, 2021 - Springer
Background DNA barcodes are a useful tool for discovering, understanding, and monitoring
biodiversity which are critical tasks at a time of rapid biodiversity loss. However, widespread …

Nanopore sequencing of long ribosomal DNA amplicons enables portable and simple biodiversity assessments with high phylogenetic resolution across broad …

H Krehenwinkel, A Pomerantz, JB Henderson… - …, 2019 - academic.oup.com
Background In light of the current biodiversity crisis, DNA barcoding is develo** into an
essential tool to quantify state shifts in global ecosystems. Current barcoding protocols often …

Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing

A Srivathsan, E Hartop, J Puniamoorthy, WT Lee… - BMC biology, 2019 - Springer
Background More than 80% of all animal species remain unknown to science. Most of these
species live in the tropics and belong to animal taxa that combine small body size with high …

DNA metabarcoding quantifies the relative biomass of arthropod taxa in songbird diets: Validation with camera‐recorded diets

YI Verkuil, M Nicolaus, R Ubels, MW Dietz… - Ecology and …, 2022 - Wiley Online Library
Ecological research is often hampered by the inability to quantify animal diets. Diet
composition can be tracked through DNA metabarcoding of fecal samples, but whether …

Three steps towards comparability and standardization among molecular methods for characterizing insect communities

E Iwaszkiewicz-Eggebrecht… - … Transactions of the …, 2024 - royalsocietypublishing.org
Molecular methods are currently some of the best-suited technologies for implementation in
insect monitoring. However, the field is develo** rapidly and lacks agreement on …

High-throughput sequencing for community analysis: the promise of DNA barcoding to uncover diversity, relatedness, abundances and interactions in spider …

SR Kennedy, S Prost, I Overcast, AJ Rominger… - … Genes and Evolution, 2020 - Springer
Large-scale studies on community ecology are highly desirable but often difficult to
accomplish due to the considerable investment of time, labor and, money required to …

Molecular diet analysis in mussels and other metazoan filter feeders and an assessment of their utility as natural eDNA samplers

S Weber, I Junk, L Brink, M Wörner… - Molecular Ecology …, 2023 - Wiley Online Library
Molecular gut content analysis is a popular tool to study food web interactions and has
recently been suggested as an alternative source for DNA‐based biomonitoring. However …

DNA metabarcoding analysis of three material types to reveal Joro spider (Trichonephila clavata) trophic interactions and web capture

EE Grabarczyk, M Querejeta, PG Tillman… - Frontiers in Ecology …, 2023 - frontiersin.org
Introduced species alter established trophic interactions and molecular analysis can resolve
changes in community structure and associated foraging links. Joro spiders (Trichonephila …