Simultaneously optimizing multiple properties of β-glucosidase Bgl6 using combined (semi-) rational design strategies and investigation of the underlying …
S Li, L Cao, X Yang, X Wu, S Xu, Y Liu - Bioresource Technology, 2023 - Elsevier
The performance of β-glucosidase during cellulose saccharification is determined by
thermostability, activity and glucose tolerance. However, conflicts between them make it …
thermostability, activity and glucose tolerance. However, conflicts between them make it …
Biochemical and Structural Analysis of a Glucose-Tolerant β-Glucosidase from the Hemicellulose-Degrading Thermoanaerobacterium saccharolyticum
β-Glucosidases (Bgls) convert cellobiose and other soluble cello-oligomers into glucose and
play important roles in fundamental biological processes, providing energy sources in living …
play important roles in fundamental biological processes, providing energy sources in living …
Elucidating the regulation of glucose tolerance in a β-glucosidase from Halothermothrix orenii by active site pocket engineering and computational analysis
Abstract β-Glucosidase catalyzes the hydrolysis of β-1, 4 linkage between two glucose
molecules in cello-oligosaccharides and is prone to inhibition by the reaction product …
molecules in cello-oligosaccharides and is prone to inhibition by the reaction product …
Beta-Glycosidase Activities of Lactobacillus spp. and Bifidobacterium spp. and The Effect of Different Physiological Conditions on Enzyme Activity
BÇ Acar, Z Yüksekdağ - Natural and Engineering Sciences, 2023 - dergipark.org.tr
In this research, food (cheese, yoghurt) and animal (chicken) origin 39 Lactobacillus spp.
and human origin (newborn faeces) three Bifidobacterium spp. were used. To designate the …
and human origin (newborn faeces) three Bifidobacterium spp. were used. To designate the …
Mechanism of imidazole inhibition of a GH1 β‐glucosidase
Imidazole is largely employed in recombinant protein purification, including GH1 β‐
glucosidases, but its effect on the enzyme activity is rarely taken into consideration …
glucosidases, but its effect on the enzyme activity is rarely taken into consideration …
Structural basis of EHEP-mediated offense against phlorotannin-induced defense from brown algae to protect akuBGL activity
X Sun, Y Ye, N Sakurai, H Wang, K Kato, J Yu, K Yuasa… - Elife, 2023 - elifesciences.org
The defensive–offensive associations between algae and herbivores determine marine
ecology. Brown algae utilize phlorotannin as their chemical defense against the predator …
ecology. Brown algae utilize phlorotannin as their chemical defense against the predator …
Homodimerization of a glycoside hydrolase family GH1 β‐glucosidase suggests distinct activity of enzyme different states
In this work, we investigated how activity and oligomeric state are related in a purified GH1 β‐
glucosidase from Spodoptera frugiperda (Sfβgly). Gel filtration chromatography coupled to a …
glucosidase from Spodoptera frugiperda (Sfβgly). Gel filtration chromatography coupled to a …
Directed evolution of enzymes
FK Tamaki - Emerging Topics in Life Sciences, 2020 - portlandpress.com
There are near-to-infinite combinations of possibilities for evolution to happen within nature,
making it yet impossible to predict how it occurs. However, science is now able to …
making it yet impossible to predict how it occurs. However, science is now able to …
Mutations close to a hub residue affect the distant active site of a GH1 β-glucosidase
The tertiary structure of proteins has been represented as a network, in which residues are
nodes and their contacts are edges. Protein structure networks contain residues, called hubs …
nodes and their contacts are edges. Protein structure networks contain residues, called hubs …
Ordinary Digestive Enzymes
Procedures to obtain reliable enzyme kinetic parameters are discussed. The classification of
enzymes regarding their function (substrate specificity and mechanism, Enzyme …
enzymes regarding their function (substrate specificity and mechanism, Enzyme …