The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has
essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular …

The appropriate timing of events that lead to chromosome segregation during mitosis and
cytokinesis is essential to prevent aneuploidy, and defects in these processes can contribute …

Analyses of protein complexes are facilitated by methods that enable the generation of
recombinant complexes via coexpression of their subunits from multigene DNA constructs …

Chemical cross-linking of reactive groups in native proteins and protein complexes in
combination with the identification of cross-linked sites by mass spectrometry has been in …

One does not often look to analytic cubism for insights into the control of the cell cycle, but
Pablo Picasso beautifully encapsulated the fundamentals when he said that “every act of …

The anaphase-promoting complex (APC/C) orchestrates progression through mitosis by
decorating cell-cycle regulators with ubiquitin chains. To nucleate chains, the APC/C links …

The anaphase-promoting complex (APC/C) is a multimeric RING E3 ubiquitin ligase that
controls chromosome segregation and mitotic exit. Its regulation by coactivator subunits …

In mitosis, the spindle assembly checkpoint (SAC) ensures genome stability by delaying
chromosome segregation until all sister chromatids have achieved bipolar attachment to the …

Motivation Due to the risk of inducing an immediate Type I (IgE-mediated) allergic response,
proteins intended for use in consumer products must be investigated for their allergenic …

Background Mass spectrometry-based proteomics enables the global identification and
quantification of proteins and their posttranslational modifications in complex biological …