Metabolically engineered Corynebacterium glutamicum for bio-based production of chemicals, fuels, materials, and healthcare products

J Becker, CM Rohles, C Wittmann - Metabolic engineering, 2018 - Elsevier
Corynebacterium glutamicum is a major workhorse in industrial biotechnology. For the past
several decades, this soil bacterium has been used to produce the amino acids l-glutamate …

Differential RNA-seq: the approach behind and the biological insight gained

CM Sharma, J Vogel - Current opinion in microbiology, 2014 - Elsevier
Highlights•RNA-seq has become the method of choice for bacterial transcriptomics.•
Differential RNA-seq (dRNA-seq) distinguishes primary and processed transcripts.•dRNA …

CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction

J Liu, M Liu, T Shi, G Sun, N Gao, X Zhao… - Nature …, 2022 - nature.com
Abstract Development of hyperproducing strains is important for biomanufacturing of
biochemicals and biofuels but requires extensive efforts to engineer cellular metabolism and …

Modeling leaderless transcription and atypical genes results in more accurate gene prediction in prokaryotes

A Lomsadze, K Gemayel, S Tang… - Genome …, 2018 - genome.cshlp.org
In a conventional view of the prokaryotic genome organization, promoters precede operons
and ribosome binding sites (RBSs) with Shine-Dalgarno consensus precede genes …

Leaderless transcripts and small proteins are common features of the mycobacterial translational landscape

SS Shell, J Wang, P Lapierre, M Mir, MR Chase… - PLoS …, 2015 - journals.plos.org
RNA-seq technologies have provided significant insight into the transcription networks of
mycobacteria. However, such studies provide no definitive information on the translational …

Microbial strain improvement by a HTP genomic engineering platform

Z Serber, EJ Dean, S Manchester, K Gora… - US Patent …, 2018 - Google Patents
2017-02-28 Assigned to Zymergen, Inc. reassignment Zymergen, Inc. ASSIGNMENT OF
ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: SHELLMAN, ERIN …

In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing

Y Wang, H Cheng, Y Liu, Y Liu, X Wen, K Zhang… - Nature …, 2021 - nature.com
Reprogramming complex cellular metabolism requires simultaneous regulation of multigene
expression. Ex-situ cloning-based methods are commonly used, but the target gene number …

[HTML][HTML] The actinobacterium Corynebacterium glutamicum, an industrial workhorse

JY Lee, YA Na, E Kim, HS Lee, P Kim - 2016 - jmb.or.kr
Starting as a glutamate producer, Corynebacterium glutamicum has played a variety of roles
in the industrial production of amino acids, one of the most important areas of white …

Enhancing astaxanthin biosynthesis and pathway expansion towards glycosylated C40 carotenoids by Corynebacterium glutamicum

VL Göttl, F Meyer, I Schmitt, M Persicke… - Scientific Reports, 2024 - nature.com
Astaxanthin, a versatile C40 carotenoid prized for its applications in food, cosmetics, and
health, is a bright red pigment with powerful antioxidant properties. To enhance astaxanthin …

Native and engineered promoters in natural product discovery

M Myronovskyi, A Luzhetskyy - Natural product reports, 2016 - pubs.rsc.org
Covers the period up to 2016 Bacterial-based natural products have long represented a
promising resource for the development of commercially relevant therapeutics, and more …