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A review of RT-PCR technologies used in veterinary virology and disease control: sensitive and specific diagnosis of five livestock diseases notifiable to the World …
B Hoffmann, M Beer, SM Reid, P Mertens… - Veterinary …, 2009 - Elsevier
Real-time, reverse transcription polymerase chain reaction (rRT-PCR) has become one of
the most widely used methods in the field of molecular diagnostics and research. The …
the most widely used methods in the field of molecular diagnostics and research. The …
Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments
JF Toussaint, C Sailleau, E Breard, S Zientara… - Journal of virological …, 2007 - Elsevier
The detection of the bluetongue virus (BTV) by conventional methods is especially difficult
and labour-intensive. Molecular diagnosis is also complex because of the high genetic …
and labour-intensive. Molecular diagnosis is also complex because of the high genetic …
Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1
AE Shaw, P Monaghan, HO Alpar, S Anthony… - Journal of virological …, 2007 - Elsevier
Since 1998, multiple strains of bluetongue virus (BTV), belonging to six different serotypes
(types 1, 2, 4, 8, 9 and 16) have caused outbreaks of disease in Europe, causing one of the …
(types 1, 2, 4, 8, 9 and 16) have caused outbreaks of disease in Europe, causing one of the …
A European field strain of bluetongue virus derived from two parental vaccine strains by genome segment reassortment
CA Batten, S Maan, AE Shaw, NS Maan, PPC Mertens - Virus research, 2008 - Elsevier
Since 1998, nine bluetongue virus (BTV) strains from serotypes 1, 2, 4, 8, 9 and 16 have
invaded Europe, killing> 2 million animals (mainly sheep). Live vaccine strains of BTV-2, 4, 9 …
invaded Europe, killing> 2 million animals (mainly sheep). Live vaccine strains of BTV-2, 4, 9 …
Vaccines and viral antigenic diversity.
JA Mumford - Revue scientifique et technique (International Office of …, 2007 - europepmc.org
Antigenic diversity among ribonucleic acid (RNA) viruses occurs as a result of rapid mutation
during replication and recombination/reassortment between genetic material of related …
during replication and recombination/reassortment between genetic material of related …
Design of primers and use of RT-PCR assays for ty** European bluetongue virus isolates: differentiation of field and vaccine strains
PPC Mertens, NS Maan, G Prasad… - Journal of General …, 2007 - microbiologyresearch.org
Bluetongue virus (BTV) is the causative agent of bluetongue, a disease of ruminant livestock
that occurs almost worldwide between latitudes 3° S and 5° N. There are 24 serotypes of …
that occurs almost worldwide between latitudes 3° S and 5° N. There are 24 serotypes of …
Rapid detection and quantitation of Bluetongue virus (BTV) using a Molecular Beacon fluorescent probe assay
Bluetongue virus (BTV) is the causative agent of Bluetongue (BT) disease in ruminant
livestock and occurs almost worldwide between latitudes 35° S and 50° N; 24 serotypes of …
livestock and occurs almost worldwide between latitudes 35° S and 50° N; 24 serotypes of …
Rapid detection and quantitation of viral hemorrhagic septicemia virus in experimentally challenged rainbow trout by real-time RT-PCR
A quantitative real-time RT-PCR (Q-RT-PCR) was developed to detect and determine the
amount of viral hemorrhagic septicemia virus (VHSV) in organs of experimentally infected …
amount of viral hemorrhagic septicemia virus (VHSV) in organs of experimentally infected …
Trategies for simultaneous detection of multiple plant viruses
D James, A Varga, V Pallas… - Canadian Journal of Plant …, 2006 - Taylor & Francis
Plants are infected by a wide range of viruses. Many cause devastation of plants and crops
resulting in significant economic losses and threats to the viability of certain horticultural and …
resulting in significant economic losses and threats to the viability of certain horticultural and …
Development and evaluation of a real-time polymerase chain reaction test for the detection of Theileria parva infections in Cape buffalo (Syncerus caffer) and cattle
Corridor disease, caused by the tick-borne protozoan parasite Theileria parva, is a
controlled disease in South Africa. The Cape buffalo is the reservoir host and uninfected …
controlled disease in South Africa. The Cape buffalo is the reservoir host and uninfected …