The history and advances in cancer immunotherapy: understanding the characteristics of tumor-infiltrating immune cells and their therapeutic implications
Immunotherapy has revolutionized cancer treatment and rejuvenated the field of tumor
immunology. Several types of immunotherapy, including adoptive cell transfer (ACT) and …
immunology. Several types of immunotherapy, including adoptive cell transfer (ACT) and …
Integrating single-cell and spatial transcriptomics to elucidate intercellular tissue dynamics
Single-cell RNA sequencing (scRNA-seq) identifies cell subpopulations within tissue but
does not capture their spatial distribution nor reveal local networks of intercellular …
does not capture their spatial distribution nor reveal local networks of intercellular …
High-plex imaging of RNA and proteins at subcellular resolution in fixed tissue by spatial molecular imaging
Resolving the spatial distribution of RNA and protein in tissues at subcellular resolution is a
challenge in the field of spatial biology. We describe spatial molecular imaging, a system …
challenge in the field of spatial biology. We describe spatial molecular imaging, a system …
The emerging landscape of spatial profiling technologies
Improved scale, multiplexing and resolution are establishing spatial nucleic acid and protein
profiling methods as a major pillar for cellular atlas building of complex samples, from …
profiling methods as a major pillar for cellular atlas building of complex samples, from …
Whole-cell segmentation of tissue images with human-level performance using large-scale data annotation and deep learning
A principal challenge in the analysis of tissue imaging data is cell segmentation—the task of
identifying the precise boundary of every cell in an image. To address this problem we …
identifying the precise boundary of every cell in an image. To address this problem we …
Resistance mechanisms to anti-PD cancer immunotherapy
The transformative success of antibodies targeting the PD-1 (programmed death 1)/B7-H1
(B7 homolog 1) pathway (anti-PD therapy) has revolutionized cancer treatment. However …
(B7 homolog 1) pathway (anti-PD therapy) has revolutionized cancer treatment. However …
Spatial omics and multiplexed imaging to explore cancer biology
Understanding intratumoral heterogeneity—the molecular variation among cells within a
tumor—promises to address outstanding questions in cancer biology and improve the …
tumor—promises to address outstanding questions in cancer biology and improve the …
Single-cell proteomics enabled by next-generation sequencing or mass spectrometry
In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …
single cells has greatly advanced our understanding of the underlying heterogeneity of …
Squidpy: a scalable framework for spatial omics analysis
Spatial omics data are advancing the study of tissue organization and cellular
communication at an unprecedented scale. Flexible tools are required to store, integrate and …
communication at an unprecedented scale. Flexible tools are required to store, integrate and …
Eleven grand challenges in single-cell data science
The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …