Origins of DNA replication in eukaryotes
Errors occurring during DNA replication can result in inaccurate replication, incomplete
replication, or re-replication, resulting in genome instability that can lead to diseases such as …
replication, or re-replication, resulting in genome instability that can lead to diseases such as …
Bridging structural and cell biology with cryo-electron microscopy
E Nogales, J Mahamid - Nature, 2024 - nature.com
Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …
structural knowledge about the macromolecules involved. For example, deciphering the …
In silico protein interaction screening uncovers DONSON's role in replication initiation
CDC45-MCM2-7-GINS (CMG) helicase assembly is the central event in eukaryotic
replication initiation. In yeast, a multi-subunit “pre-loading complex”(pre-LC) accompanies …
replication initiation. In yeast, a multi-subunit “pre-loading complex”(pre-LC) accompanies …
The structural mechanism of dimeric DONSON in replicative helicase activation
MA Cvetkovic, P Passaretti, A Butryn… - Molecular Cell, 2023 - cell.com
The MCM motor of the replicative helicase is loaded onto origin DNA as an inactive double
hexamer before replication initiation. Recruitment of activators GINS and Cdc45 upon S …
hexamer before replication initiation. Recruitment of activators GINS and Cdc45 upon S …
DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans
Assembly of the CMG (CDC-45–MCM-2-7–GINS) helicase is the key regulated step during
eukaryotic DNA replication initiation. Until now, it was unclear whether metazoa require …
eukaryotic DNA replication initiation. Until now, it was unclear whether metazoa require …
The human pre-replication complex is an open complex
J Li, J Dong, W Wang, D Yu, X Fan, YC Hui, CSK Lee… - Cell, 2023 - cell.com
In eukaryotes, DNA replication initiation requires assembly and activation of the
minichromosome maintenance (MCM) 2–7 double hexamer (DH) to melt origin DNA …
minichromosome maintenance (MCM) 2–7 double hexamer (DH) to melt origin DNA …
A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance
Faithful transfer of parental histones to newly replicated daughter DNA strands is critical for
inheritance of epigenetic states. Although replication proteins that facilitate parental histone …
inheritance of epigenetic states. Although replication proteins that facilitate parental histone …
Synergism between CMG helicase and leading strand DNA polymerase at replication fork
The replisome that replicates the eukaryotic genome consists of at least three engines: the
Cdc45-MCM-GINS (CMG) helicase that separates duplex DNA at the replication fork and …
Cdc45-MCM-GINS (CMG) helicase that separates duplex DNA at the replication fork and …
Mechanism of eukaryotic origin unwinding is a dual helicase DNA shearing process
LD Langston, RE Georgescu… - Proceedings of the …, 2023 - National Acad Sciences
DNA replication in all cells begins with the melting of base pairs at the duplex origin to allow
access to single-stranded DNA templates which are replicated by DNA polymerases. In …
access to single-stranded DNA templates which are replicated by DNA polymerases. In …
CMG helicase disassembly is essential and driven by two pathways in budding yeast
C Polo Rivera, TD Deegan, KPM Labib - The EMBO Journal, 2024 - embopress.org
The CMG helicase is the stable core of the eukaryotic replisome and is ubiquitylated and
disassembled during DNA replication termination. Fungi and animals use different enzymes …
disassembled during DNA replication termination. Fungi and animals use different enzymes …