Precursor messenger RNA (pre-mRNA) splicing is a critical step in the posttranscriptional
regulation of gene expression, providing significant expansion of the functional proteome of …

The programmes that direct an organism's development and maintenance are encoded in its
genome. Decoding of this information begins with regulated transcription of genomic DNA …

Pseudouridine is a modified nucleotide that is prevalent in human mRNAs and is
dynamically regulated. Here, we investigate when in their life cycle mRNAs become …

Understanding the biologic role of N 6-methyladenosine (m6A) RNA modifications in mRNA
requires an understanding of when and where in the life of a pre-mRNA transcript the …

Maturation of mRNA precursors often occurs simultaneously with their synthesis by RNA
polymerase II (Pol II). The co-transcriptional nature of mRNA processing has permitted the …

Understanding how splicing events are coordinated across numerous introns in metazoan
RNA transcripts requires quantitative analyses of transient RNA processing events in living …

Intron retention has long been an exemplar of regulated splicing with case studies of
individual events serving as models that provided key mechanistic insights into the process …

Deep sequencing of embryonic stem cell RNA revealed many specific internal introns that
are significantly more abundant than the other introns within polyadenylated transcripts; we …

How Polycomb repressive complex 2 (PRC2) is regulated by RNA remains an unsolved
problem. Although PRC2 binds G-tracts with the potential to form RNA G-quadruplexes …

Summary mRNA is thought to predominantly reside in the cytoplasm, where it is translated
and eventually degraded. Although nuclear retention of mRNA has a regulatory potential, it …