Production of recombinant proteins by microbes and higher organisms

AL Demain, P Vaishnav - Biotechnology advances, 2009 - Elsevier
Large proteins are usually expressed in a eukaryotic system while smaller ones are
expressed in prokaryotic systems. For proteins that require glycosylation, mammalian cells …

Statistical approaches to maximize recombinant protein expression in Escherichia coli: a general review

CP Papaneophytou, G Kontopidis - Protein expression and purification, 2014 - Elsevier
The supply of many valuable proteins that have potential clinical or industrial use is often
limited by their low natural availability. With the modern advances in genomics, proteomics …

[PDF][PDF] 2012

N Rodríguez - HEAL: Humanism Evolving through Arts and …, 2012 - journals.flvc.org
Sarah Adopted Parents 2012 Page 1 The Florida State University College of Medicine 17 16
HEALVol 4, 2012 I have pictured this moment—finally getting the opportunity to let those …

Production and Purification of Recombinant Proteins from Escherichia coli

NK Tripathi - ChemBioEng Reviews, 2016 - Wiley Online Library
Abstract Development of successful production strategies for recombinant proteins is a
challenging task due to problems associated with the scale‐up of bioprocesses. Recent …

Interferon-based biopharmaceuticals: Overview on the production, purification, and formulation

LS Castro, GS Lobo, P Pereira, MG Freire, MC Neves… - Vaccines, 2021 - mdpi.com
The advent of biopharmaceuticals in modern medicine brought enormous benefits to the
treatment of numerous human diseases and improved the well-being of many people …

Experimental study on the effects of air blowing and irradiance intensity on the performance of photovoltaic modules, using central composite design

AH Shiravi, M Firoozzadeh, M Lotfi - Energy, 2022 - Elsevier
Photovoltaic (PV) technology plays an important role in the progressing trend of using
renewable energies in the world. Yet the negative impact of a cell's temperature rise on its …

[HTML][HTML] The goldilocks approach: A review of employing design of experiments in prokaryotic recombinant protein production

A Uhoraningoga, GK Kinsella, GT Henehan, BJ Ryan - Bioengineering, 2018 - mdpi.com
The production of high yields of soluble recombinant protein is one of the main objectives of
protein biotechnology. Several factors, such as expression system, vector, host, media …

Optimization of fermentation conditions for recombinant human interferon beta production by Escherichia coli using the response surface methodology

MH Morowvat, V Babaeipour… - Jundishapur journal …, 2015 - pmc.ncbi.nlm.nih.gov
Background: The periplasmic overexpression of recombinant human interferon beta (rhIFN-
β)-1b using a synthetic gene in Escherichia coli BL21 (DE3) was optimized in shake flasks …

Comparison of the estimation capabilities of response surface methodology and artificial neural network for the optimization of recombinant lipase production by E. coli …

R Nelofer, RN Ramanan… - Journal of industrial …, 2012 - academic.oup.com
Response surface methodology (RSM) and artificial neural network (ANN) were used to
optimize the effect of four independent variables, viz. glucose, sodium chloride (NaCl) …

[HTML][HTML] Optimization of expression and purification of HSPA6 protein from Camelus dromedarius in E. coli

A Malik, AM Alsenaidy, M Elrobh, W Khan… - Saudi Journal of …, 2016 - Elsevier
The HSPA6, one of the members of large family of HSP70, is significantly up-regulated and
has been targeted as a biomarker of cellular stress in several studies. Herein, conditions …