Programmable gene-editing tools have transformed the life sciences and have shown
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …

CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Deep Learning (DL) has recently enabled unprecedented advances in one of the grand
challenges in computational biology: the half-century-old problem of protein structure …

Prime editing enables the installation of virtually any combination of point mutations, small
insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) …

Feature attributions based on the Shapley value are popular for explaining machine
learning models. However, their estimation is complex from both theoretical and …

The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

Prime editing is a versatile genome editing tool but requires experimental optimization of the
prime editing guide RNA (pegRNA) to achieve high editing efficiency. Here we conducted a …

Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR–
Cas)-mediated genome editing has revolutionized biomedical research and will likely …

Prime editing enables a wide variety of precise genome edits in living cells. Here we use
protein evolution and engineering to generate prime editors with reduced size and improved …

Prime editing (PE) applications are limited by low editing efficiency. Here we show that
designing prime binding sites with a melting temperature of 30° C leads to optimal …