Avidin-biotin interaction is one of the strongest non-covalent interactions in the nature.
Avidin and its analogues have therefore been extensively utilized as probes and affinity …

Background Egg is one of the most nutritious foods that is easily available and it has
become a favorite source of major nutrients like lipids and proteins around the world …

An immunoassay is an analytical test method in which analyte quantitation is based on
signal responses generated as a consequence of an antibody–antigen interaction. They are …

Previous studies tracking AMPA receptor (AMPAR) diffusion at synapses observed a large
mobile extrasynaptic AMPAR pool. Using super-resolution microscopy, we examined how …

The high affinity (KD~ 10− 15 M) of biotin for avidin and streptavidin is the essential
component in a multitude of bioassays with many experiments using biotin modifications to …

Although streptavidin's high affinity for biotin has made it a widely used and studied binding
protein and labeling tool, its tetrameric structure may interfere with some assays. A …

Isothermal titration calorimetry (ITC) is a fast, accurate and label‐free method for measuring
the thermodynamics and binding affinities of molecular associations in solution. Because the …

Streptavidin (SA), and other related proteins, has been isolated from a wide range of
organisms, including bacteria, fungi, frogs, fish, and birds. Although their original function is …

Rhizavidin, from the proteobacterium Rhizobium etli, exhibits high affinity towards biotin but
maintains an inherent dimeric quaternary structure and thus, differs from all other known …

Background Photoimmunotherapy (PIT) employing antibody-photosensitizer conjugates is a
promising treatment for cancer. However, the fixed antigen specificity severely limits the …