Single-molecule FRET for probing nanoscale biomolecular dynamics

D Nettels, N Galvanetto, MT Ivanović, M Nüesch… - Nature Reviews …, 2024 - nature.com
Single-molecule spectroscopy is a powerful method for studying the physics of molecular
systems, particularly biomolecules, such as proteins and nucleic acids. By avoiding …

Recent advances in the label-free characterization of exosomes for cancer liquid biopsy: from scattering and spectroscopy to nanoindentation and nanodevices

R Di Santo, S Romanò, A Mazzini, S Jovanović… - Nanomaterials, 2021 - mdpi.com
Exosomes (EXOs) are nano-sized vesicles secreted by most cell types. They are abundant
in bio-fluids and harbor specific molecular constituents from their parental cells. Due to these …

Structural heterogeneity of the ion and lipid channel TMEM16F

Z Ye, N Galvanetto, L Puppulin, S Pifferi… - Nature …, 2024 - nature.com
Abstract Transmembrane protein 16 F (TMEM16F) is a Ca2+-activated homodimer which
functions as an ion channel and a phospholipid scramblase. Despite the availability of …

Application of self-organizing maps to AFM-based viscoelastic characterization of breast cancer cell mechanics

A Weber, MDM Vivanco, JL Toca-Herrera - Scientific Reports, 2023 - nature.com
Cell mechanical properties have been proposed as label free markers for diagnostic
purposes in diseases such as cancer. Cancer cells show altered mechanical phenotypes …

Advancing High-Throughput Cellular Atomic Force Microscopy with Automation and Artificial Intelligence

O Thomas--Chemin, S Janel, Z Boumehdi, C Séverac… - ACS …, 2025 - ACS Publications
Atomic force microscopy (AFM) has reached a significant level of maturity in biology,
demonstrated by the diversity of modes for obtaining not only topographical images but also …

Iterative Machine Learning for Classification and Discovery of Single-Molecule Unfolding Trajectories from Force Spectroscopy Data

V Doffini, H Liu, Z Liu, MA Nash - Nano Letters, 2023 - ACS Publications
We report the application of machine learning techniques to expedite classification and
analysis of protein unfolding trajectories from force spectroscopy data. Using kernel …

[HTML][HTML] Nanomechanical Characterization of Soft Nanomaterial Using Atomic Force Microscopy

DL Chi, S Park - Materials Today Bio, 2025 - Elsevier
Atomic force microscopy (AFM) is a promising method for generating high-spatial-resolution
images, providing insightful perspectives on the nanomechanical attributes of soft matter …

Unfolding and identification of membrane proteins in situ

N Galvanetto, Z Ye, A Marchesi, S Mortal, S Maity… - Elife, 2022 - elifesciences.org
Single-molecule force spectroscopy (SMFS) uses the cantilever tip of an atomic force
microscope (AFM) to apply a force able to unfold a single protein. The obtained force …

Unfolding and identification of membrane proteins in situ

G Nicola, Y Zhongjie, M Arin, M Simone, M Sourav… - eLife, 2022 - search.proquest.com
Single-molecule force spectroscopy (SMFS) uses the cantilever tip of an atomic force
microscope (AFM) to apply a force able to unfold a single protein. The obtained force …

Biophysical exploration of membrane-protein interactions in Alzheimer's Disease

A Karkisaval Ganapati - 2023 - escholarship.org
Alzheimer's disease (AD) is a devastating neurodegenerative disorder affecting millions of
people worldwide and the number is projected to rapidly increase in the coming years. AD …