Cellular plasticity in reprogramming, rejuvenation and tumorigenesis: a pioneer TF perspective

A Huyghe, A Trajkova, F Lavial - Trends in cell biology, 2024 - cell.com
The multistep process of in vivo reprogramming, mediated by the transcription factors (TFs)
Oct4, Sox2, Klf4, and c-Myc (OSKM), holds great promise for the development of …

Engineering considerations of iPSC-based personalized medicine

S Park, Y Gwon, SA Khan, KJ Jang, J Kim - Biomaterials Research, 2023 - spj.science.org
Personalized medicine aims to provide tailored medical treatment that considers the clinical,
genetic, and environmental characteristics of patients. iPSCs have attracted considerable …

Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin

M Tedesco, F Giannese, D Lazarević, V Giansanti… - Nature …, 2022 - nature.com
Recent efforts have succeeded in surveying open chromatin at the single-cell level, but high-
throughput, single-cell assessment of heterochromatin and its underlying genomic …

Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair

M Kovatcheva, E Melendez, D Chondronasiou… - Nature …, 2023 - nature.com
Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a
therapeutic strategy for tissue regeneration and rejuvenation, but little is known about its …

H3K36 methylation maintains cell identity by regulating opposing lineage programmes

MS Hoetker, M Yagi, B Di Stefano, J Langerman… - Nature cell …, 2023 - nature.com
The epigenetic mechanisms that maintain differentiated cell states remain incompletely
understood. Here we employed histone mutants to uncover a crucial role for H3K36 …

A fast chemical reprogramming system promotes cell identity transition through a diapause-like state

X Chen, Y Lu, L Wang, X Ma, J Pu, L Lin, Q Deng… - Nature Cell …, 2023 - nature.com
Cellular reprogramming by only small molecules holds enormous potentials for regenerative
medicine. However, chemical reprogramming remains a slow process and labour intensive …

Dissecting OCT4 defines the role of nucleosome binding in pluripotency

GA Roberts, B Ozkan, I Gachulincová, MR O'Dwyer… - Nature cell …, 2021 - nature.com
Pioneer transcription factors such as OCT4 can target silent genes embedded in
nucleosome-dense regions. How nucleosome interaction enables transcription factors to …

STARR-seq identifies active, chromatin-masked, and dormant enhancers in pluripotent mouse embryonic stem cells

T Peng, Y Zhai, Y Atlasi, M Ter Huurne, H Marks… - Genome biology, 2020 - Springer
Background Enhancers are distal regulators of gene expression that shape cell identity and
control cell fate transitions. In mouse embryonic stem cells (mESCs), the pluripotency …

The emergence of Sox and POU transcription factors predates the origins of animal stem cells

Y Gao, DS Tan, M Girbig, H Hu, X Zhou, Q **e… - Nature …, 2024 - nature.com
Stem cells are a hallmark of animal multicellularity. Sox and POU transcription factors are
associated with stemness and were believed to be animal innovations, reported absent in …

Nucleosome fibre topology guides transcription factor binding to enhancers

MR O'Dwyer, M Azagury, K Furlong, A Alsheikh… - Nature, 2024 - nature.com
Cellular identity requires the concerted action of multiple transcription factors (TFs) bound
together to enhancers of cell-type-specific genes. Despite TFs recognizing specific DNA …