Objective The apolipoprotein E (APOE) E4 isoform is the strongest genetic risk factor for
sporadic Alzheimer disease (AD). Although APOE is predominantly expressed by astrocytes …

Current experimental models of blast injuries used to study blast-induced neurotrauma
(BINT) vary widely, which makes the comparison of the experimental results extremely …

Background Accurate interpretation of quantitative PCR (qPCR) data requires normalization
using constitutively expressed reference genes. Ribosomal RNA is often used as a …

Genes that are stably expressed during development or in response to environmental
changes are essential for accurate normalization in qRT–PCR experiments. To prevent …

Overexpression or pharmacological activation of SIRT1 has been shown to extend the
lifespan of mice and protect against aging-related diseases. Here we show that …

Background As one of the environmental risk factors for human health, atmospheric fine
particulate matter (PM2. 5) contributes to cognitive deterioration in addition to respiratory …

Background Quantification and normalization of RT-qPCR data critically depends on the
expression of so called reference genes. Our goal was to develop a strategy for the selection …

Gene expression analysis is increasingly important in biological research, with reverse
transcription–quantitative PCR (RT–qPCR) becoming the method of choice for high …

The use of reference genes as internal controls is commonly accepted as the most
appropriate normalization strategy in quantitative real-time PCR (qPCR) assays. However …

Quantitative real-time polymerase chain reaction (qPCR), as one of the most sensitive and
precise gene expression analysis methods, is frequently used to validate data obtained in …