Glioblastoma (GBM) is the most invasive and deadliest brain cancer in adults. Its inherent
heterogeneity has been designated as the main cause of treatment failure. Thus, a deeper …

The CRISPR genome editing technology has revolutionized the way gene function is
studied. Genome editing can be achieved in single genes or for thousands of genes …

Abstract Single-cell RNA-Seq (scRNA-Seq) profiles gene expression of individual cells.
Recent scRNA-Seq datasets have incorporated unique molecular identifiers (UMIs). Using …

Background Single-cell transcriptomics is rapidly advancing our understanding of the
cellular composition of complex tissues and organisms. A major limitation in most analysis …

Background The rapid development of single-cell RNA-sequencing (scRNA-seq)
technologies has led to the emergence of many methods for removing systematic technical …

A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read
and long-read (nanopore) sequencing together with a new computational pipeline …

Background Despite extensive molecular characterization, we lack a comprehensive
understanding of lineage identity, differentiation, and proliferation in high-grade gliomas …

Chronic inflammation is often associated with the development of tissue fibrosis, but how
mesenchymal cell responses dictate pathological fibrosis versus resolution and healing …

Dropout events in single-cell RNA sequencing (scRNA-seq) cause many transcripts to go
undetected and induce an excess of zero read counts, leading to power issues in differential …

Genes associated with risk for brain disease exhibit characteristic expression patterns that
reflect both anatomical and cell type relationships. Brain-wide transcriptomic patterns of …